Department of Microbiology and Immunology, Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Mol Biol Rep. 2019 Dec;46(6):5703-5712. doi: 10.1007/s11033-019-05004-1. Epub 2019 Jul 29.
Helicobacter pylori (H. pylori) has been shown to be one of the leading causes of peptic ulcer diseases (PUDs) and gastritis. T helper-22 (Th22) cells and its most important cytokine, interleukin-22 (IL-22) are importantly active in inflammation and inflammatory tissues. Since inflammation is one of the main attributes of infection caused by H. pylori and resulting complications (gastritis and gastrointestinal ulcer), this study was designed to evaluate the Th22 cells count and the IL-22 protein expression in people suffering from PUD and gastritis. The present study was conducted on 55 patients with gastritis, 47 patients with PUD and 48 uninfected subjects. After preparation of section and extraction of protein from antral biopsies, immunohistochemistry and western blot methods were used to evaluate the Th22 cells and IL-22 protein expression level, respectively. According to findings, the Th22 cells count and the IL-22 protein expression level in the infected subjects were siginficantly more than in the uninfected subjects. It should be noted that the Th22 cells count and the IL-22 protein expression level in the infected subjects with PUD were significantly greater than those in the infected subjects with gastritis. In addition, the Th22 cells count had positive correlation with the density of H. pylori, chronic inflammation score and acute inflammatory score in the infected subjects with PUD. The Th22 cells count had positive correlation with the Th17 cells count and inverse correlation with the Treg cells count in the infected subjects with PUD and gastritis. Our data demonstrated that abnormal hyper-activation of Th22 cells as well as its correlation with the Th17 cells during infection caused by H. pylori might damage tissues through immunopathological responses.
幽门螺杆菌（H. pylori）已被证实是消化性溃疡病（PUD）和胃炎的主要病因之一。辅助性 T 细胞 22（Th22）及其最重要的细胞因子白细胞介素-22（IL-22）在炎症和炎症组织中具有重要的活性。由于炎症是由 H. pylori 引起的感染及其导致的并发症（胃炎和胃肠道溃疡）的主要特征之一，因此本研究旨在评估患有 PUD 和胃炎的人群中 Th22 细胞计数和 IL-22 蛋白表达。本研究共纳入 55 例胃炎患者、47 例 PUD 患者和 48 例未感染者。在制备胃窦活检切片和提取蛋白质后，分别采用免疫组织化学和 Western blot 方法评估 Th22 细胞和 IL-22 蛋白表达水平。结果显示，感染组的 Th22 细胞计数和 IL-22 蛋白表达水平显著高于未感染组。值得注意的是，感染 PUD 的患者的 Th22 细胞计数和 IL-22 蛋白表达水平显著高于感染胃炎的患者。此外，感染 PUD 的患者的 Th22 细胞计数与 H. pylori 的密度、慢性炎症评分和急性炎症评分呈正相关。感染 PUD 和胃炎的患者的 Th22 细胞计数与 Th17 细胞计数呈正相关，与 Treg 细胞计数呈负相关。我们的数据表明，在 H. pylori 感染期间 Th22 细胞的异常过度激活及其与 Th17 细胞的相关性可能通过免疫病理反应对组织造成损害。
