Ray Swapan K
Department of Pathology, Microbiology, and Immunology, University of South Carolina School of Medicine, Columbia, SC, USA.
Methods Mol Biol. 2019;2019:257-273. doi: 10.1007/978-1-4939-9585-1_18.
Detection of any decrease in telomerase activity in cancer cells and tumor tissues is an important part in assessing overall therapeutic outcomes of a treatment agent in the laboratory and clinical settings. Almost 85% of cancers have activation of telomerase activity that promotes cell proliferation and discourages differentiation to sustain growth of the cancers. Retinoids are highly regarded as the anti-proliferation and pro-differentiation agents that cause down regulation of telomerase activity in the cancer cells. Two (nonradioactive and radioactive) telomeric repeat amplification protocol (TRAP) assays are optimized and fully described for detection of the diminished or abolished telomerase activity in a very low amount of protein extracts from cancer cells after treatment with a natural retinoid or a synthetic retinoid. These highly optimized and improved nonradioactive and radioactive TRAP assays can also be used for determining the presence or absence of telomerase activity in a small amount of any tumor tissue. The results from these TRAP assays can also help decide appropriate therapeutic options for the cancers with or without telomerase activity.
检测癌细胞和肿瘤组织中端粒酶活性的任何降低，是在实验室和临床环境中评估治疗药物总体治疗效果的重要部分。几乎85%的癌症都有端粒酶活性的激活，这促进细胞增殖并抑制分化，以维持癌症的生长。维甲酸被高度视为抗增殖和促分化剂，可导致癌细胞中端粒酶活性下调。两种（非放射性和放射性）端粒重复序列扩增法（TRAP）经过优化并得到全面描述，用于检测在用天然维甲酸或合成维甲酸处理后的癌细胞极少量蛋白质提取物中端粒酶活性的降低或丧失。这些高度优化和改进的非放射性和放射性TRAP检测方法，也可用于确定任何少量肿瘤组织中端粒酶活性的有无。这些TRAP检测的结果还可帮助为有或无端粒酶活性的癌症确定合适的治疗方案。
