Tan Xiao-Ming, Yu Li-Ying, Tang Hong-Zhen, Zhao Li-Chun, Zhou Ya-Qin
Guangxi University of Chinese Medicine Nanning 530200, China.
Guangxi Botanical Garden of Medicinal Plant Nanning 530023, China.
Zhongguo Zhong Yao Za Zhi. 2019 Jun;44(11):2226-2230. doi: 10.19540/j.cnki.cjcmm.20190301.012.
Using the White as basic medium, the effects of the exogenous IBA and endophytic fungal elicitor on the growth of in vitro roots cultures of Dysosma versipellis and production of podophyllotoxin were investigated in this study. The results showed that the IBA and the endophytic fungus Zasmidium syzygii elicitor could increase the content of podophyllotoxin of in vitro roots of D. versipellis after 3 weeks. The White medium added with 3 mg·L(-1) IBA induced the highest increase of podophyllotoxin(1 830.86 μg·g(-1)), which was 2.07 folds greater than the control, and followed by 1.5 mg·L(-1) IBA, fungal elicitor, 1 mg·L(-1) IBA, 0.5 mg·L(-1) IBA and 4.5 mg·L(-1) IBA, which was 1.82, 1.71, 1.63, 1.43 and 1.1 folds greater than the control, respectively. The results also showed that the growth of roots was certain positively correlated with the change of IBA concentration. Therefore, 3 mg·L(-1) IBA was the most suitable for the production of podophyllotoxin in the in vitro roots of D. versipellis, and the stimulating effect of Z. syzygii fungal elicitor was between 1.5 mg·L(-1) and 1 mg·L~(-1) IBA, which was a potential natural elicitor to induce the accumulation of podophyllotoxin in future production.
以White培养基为基础培养基，研究了外源吲哚丁酸（IBA）和内生真菌诱导子对八角莲离体根培养物生长及鬼臼毒素产生的影响。结果表明，IBA和内生真菌合轴链格孢诱导子处理3周后可提高八角莲离体根中鬼臼毒素的含量。添加3 mg·L⁻¹ IBA的White培养基诱导的鬼臼毒素含量增加最高（1 830.86 μg·g⁻¹），比对照高2.07倍，其次是1.5 mg·L⁻¹ IBA、真菌诱导子、1 mg·L⁻¹ IBA、0.5 mg·L⁻¹ IBA和4.5 mg·L⁻¹ IBA，分别比对照高1.82倍、1.71倍、1.63倍、1.43倍和1.1倍。结果还表明，根的生长与IBA浓度的变化呈一定的正相关。因此，3 mg·L⁻¹ IBA最适合八角莲离体根中鬼臼毒素的生产，合轴链格孢真菌诱导子的刺激效果介于1.5 mg·L⁻¹和1 mg·L⁻¹ IBA之间，是未来生产中诱导鬼臼毒素积累的潜在天然诱导子。
