Yang Qing-Xin, Lyu Hong-Jun, Wang Xiao-Bo, Jiang Lei, Zeng Nan
the Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine,Key Laboratory of Systematic Research,Development and Utilization of Chinese Medicine Resources in Sichuan Province-Key Laboratory Breeding Base of Co-founded by Sichuan Province and MOST,Pharmacy College,Chengdu University of Traditional Chinese Medicine Chengdu 611137,China.
Department of Neurology,Kaifeng Central Hospital Kaifeng 475000,China.
Zhongguo Zhong Yao Za Zhi. 2019 Jul;44(13):2691-2700. doi: 10.19540/j.cnki.cjcmm.20190416.404.
Guizhi Decoction is a resolving agent,which is a classic prescription for traditional Chinese medicine. It is effective in the treatment of sepsis in clinical practice. However,due to the complexity of the prescription,its anti-sepsis mechanism is difficult to be clarified. The " Cinnamomi Ramulus-Paeoniae Radix Alba" drug pair,as the classic compatibility for medicinal and medicinal herbs,is the core of Guizhi Decoction. In this study,Cinnamomi Ramulus-Paeoniae Radix Alba drug pair was used as the research object and the molecular mechanism of its treatment of sepsis was investigated by analyzing the chemical compositions with integrative pharmacology platform( TCMIP,http://www.tcmip.cn/),predicting disease target,analyzing gene function and pathway of " Cinnamomi Ramulus-Paeoniae Radix Alba" in treatment of sepsis,and establishing a multi-dimensional network relationship of " Chinese medicine-chemical components-core targets-key pathways". The prediction results of " Cinnamomi Ramulus-Paeoniae Radix Alba" drug pair showed that its anti-sepsis effect was associated with 45 active components,and the active components played an anti-sepsis role through multiple targets and pathways,involving inflammatory targets such as PF4,MyD88,TLR4,BDKRB2,CD14,and NOS3. The sepsis was relieved mainly by regulating Toll like signaling pathway,Fox O signaling pathway,chemokines signaling pathway,thyroid and insulin endocrine signaling pathways and biological processes. This study provides a scientific basis for further development of Cinnamomi Ramulus-Paeoniae Radix Alba drug pair and Guizhi Decoction against sepsis.
桂枝汤是一种解表剂，是中医经典方剂。在临床实践中，它对脓毒症的治疗有效。然而，由于该方剂成分复杂，其抗脓毒症机制难以阐明。“桂枝-白芍”药对作为中药与中药的经典配伍，是桂枝汤的核心。本研究以“桂枝-白芍”药对为研究对象，通过综合药理学平台（TCMIP，http://www.tcmip.cn/）分析其化学成分、预测疾病靶点、分析“桂枝-白芍”治疗脓毒症的基因功能和通路，并建立“中药-化学成分-核心靶点-关键通路”的多维网络关系，探讨其治疗脓毒症的分子机制。“桂枝-白芍”药对的预测结果表明，其抗脓毒症作用与45种活性成分有关，这些活性成分通过多个靶点和通路发挥抗脓毒症作用，涉及PF4、MyD88、TLR4、BDKRB2、CD14和NOS3等炎症靶点。脓毒症主要通过调节Toll样信号通路、Fox O信号通路、趋化因子信号通路、甲状腺和胰岛素内分泌信号通路及生物学过程得到缓解。本研究为进一步开发“桂枝-白芍”药对和桂枝汤抗脓毒症提供了科学依据。
