Zhang Xue, Wang Yun, Wang Qing-Hao, Wang Guo-You, Liu De-Peng, Ma Yin-Lian, Song Yan, Zhang Yu-Lian, Lyu Ting-Ting, Zhang Cun
Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China.
Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China College of Pharmacy,Henan University of Chinese Medicine Zhengzhou 450008,China.
Zhongguo Zhong Yao Za Zhi. 2019 Jul;44(13):2792-2798. doi: 10.19540/j.cnki.cjcmm.20190321.309.
In this experiment,the gradation analysis method was used to evaluate the quality of different pieces of Gardeniae Fructus through the extraction rate difference and the difference analysis of the main components in the extract. In this experiment cold-dip and hot-dip methods were used to compare the yield of Gardeniae Fructus extract and the content of chemical constituents with water,25%,50%,75% and 95% ethanol fractions. By weighted calculation,the optimal extraction method of Gardeniae Fructus was determined,and this was verified by practical application. RESULTS:: showed that for the water-soluble extract,cold dip method was better than the hot dip method; and for alcohol-soluble extract,75% ethanol under cold dip method was best. The verification results showed that water-soluble extracts under cold dip methods could be used to significantly distinguish the raw Gardeniae Fructus( GF) and processed( stir-baked) GF( GFP) collected from the market. Meanwhile,this method could be also used to distinguish the same batch of GF,GFP and carbonized GF( GFC) with significant differences,respectively( P<0. 05). Ethanol-soluble extract can be used to clearly distinguish GFP and GFC pieces in the same batch( P<0. 05). The results of content determination showed that the variation coefficient of components in GF processed products was higher than that in extracts,and the content of hydroxygeniposide was the most significant component between GF and its processed products. It is suggested that the method of water-soluble extract of GF and the determination of the content of gardoside should be combined together to evaluate the quality of GF and its heat processed products.
在本实验中，采用梯度分析方法，通过不同栀子饮片提取物得率差异及提取物中主要成分的差异分析来评价其质量。本实验采用冷浸法和热浸法比较栀子以水、25%、50%、75%和95%乙醇为溶剂的提取物得率及化学成分含量。通过加权计算，确定栀子的最佳提取方法，并通过实际应用进行验证。结果表明：对于水溶性提取物，冷浸法优于热浸法；对于醇溶性提取物，冷浸法中75%乙醇效果最佳。验证结果表明，冷浸法得到的水溶性提取物可显著区分市售生栀子（GF）和炮制品（炒栀子，GFP）。同时，该方法也可分别显著区分同一批次的GF、GFP和炭栀子（GFC）（P<0.05）。醇溶性提取物可清晰区分同一批次的GFP和GFC饮片（P<0.05）。含量测定结果表明，栀子炮制品中各成分的变异系数高于提取物，且京尼平苷含量是栀子及其炮制品之间差异最显著的成分。建议将栀子水溶性提取物方法与栀子苷含量测定相结合，以评价栀子及其炮制产品的质量。
