Hammou Rahma Ait, Benhessou Mustapha, Bouziyane Amal, Hassou Najwa, Benhchekroun Mohammed Nabil, Bessi Hlima, Ennaji Moulay Mustapha
Laboratory of Virology,Microbiology, Quality and Biotechnologies/Eco-toxicology and biodiversity,faculty of Sciences and Techniques, Mohammedia,University Hassan II of Casablanca,BP:146 Mohammedia 20650, Morocco.
Universite Mohamed VI des Sciences de la sante of Casablanca, Morocco.
Bioinformation. 2019 Feb 3;15(1):55-60. doi: 10.6026/97320630015055. eCollection 2019.
Epithelial Ovarian cancer (EOC) although rare is the most lethal gynecological cancer in women worldwide. Despite its high prevalence few studies have been performed to evaluate the prevalence and determinants of HPV infection worldwide. The aim of the present study was to investigate the presence of HPV-DNA in Moroccan patients with EOC using PCR among women in Casablanca, and to examine the prevalence of some HPV genotypes in Moroccan population. We performed a study of HPV detection on Fresh biopsies of 70 epithelial ovarian cancer patients. PCR was realized using the MY09/11 and GP5+/6+ primers. Genotyping of HPV was performed by PCR typespecific for HPV 6, 11, 16, 18, 31, and 33.Data was statistically analyzed using SPSS software. Hence, the mean age was 48.9 years (range,21-76 years). Serous adeno carcinoma (75.71%) and stage III of the disease represent the majority of cases. eight patients were HPV positive (11.42%).Results of HPV genotyping revealed predominance of two genotypes: HPV 16 (87.5%) and HPV 31(12.5).No co-infection identified. Approximately 75% of positive cases had a serous cystadeno carcinoma and more than 62,5% had FIGO advanced stage (III or IV).Our study showed that high-risk HPV infection could play a major role among patients with EOC in Morocco.
上皮性卵巢癌（EOC）虽然罕见，但却是全球女性中最致命的妇科癌症。尽管其发病率很高，但在全球范围内，很少有研究评估人乳头瘤病毒（HPV）感染的患病率及其决定因素。本研究的目的是使用聚合酶链反应（PCR）检测卡萨布兰卡女性摩洛哥EOC患者中HPV-DNA的存在情况，并检测摩洛哥人群中某些HPV基因型的患病率。我们对70例上皮性卵巢癌患者的新鲜活检组织进行了HPV检测研究。使用MY09/11和GP5+/6+引物进行PCR。通过针对HPV 6、11、16、18、31和33的型特异性PCR对HPV进行基因分型。使用SPSS软件对数据进行统计分析。因此，平均年龄为48.9岁（范围为21 - 76岁）。浆液性腺癌（75.71%）和疾病的III期占大多数病例。8例患者HPV呈阳性（11.42%）。HPV基因分型结果显示两种基因型占主导：HPV 16（87.5%）和HPV 31（12.5%）。未发现合并感染。大约75%的阳性病例患有浆液性囊腺癌，超过62.5%的病例处于国际妇产科联盟（FIGO）晚期（III期或IV期）。我们的研究表明，高危HPV感染在摩洛哥EOC患者中可能起主要作用。
