Laboratory for Molecular Genetics, Institute for Oncology and Radiology of Serbia, Pasterova 14, 11 000, Belgrade, Serbia.
Arch Gynecol Obstet. 2012 Sep;286(3):723-8. doi: 10.1007/s00404-012-2367-6. Epub 2012 May 9.
The objective of this study was to investigate the presence of human papillomavirus (HPV) infection in ovarian carcinoma samples from Serbian women and correlate them with clinicopathological characteristics of disease and patients' characteristics.
Fifty-four ovarian carcinoma patients were included in the study. DNA was isolated by salting out method from tumor tissue obtained after surgical treatment. Presence of HPV infection was detected through polymerase chain reaction amplification of a 150-bp fragment of L1 viral gene by GP5+/GP6+ primers. HPV genotyping was performed by DNA sequencing.
HPV DNA was present in 4/54 (7.4 %) ovarian carcinomas. All HPV-positive tumors contained high-risk HPV16 type. HPV infection was more common in advanced opposite to localized disease. The median age of diagnosis of disease varied from 57 years for patients with HPV infection to 59 years for patients without HPV infection.
Our results indicate that HPV infection may play a limited role in ovarian carcinogenesis.
本研究旨在调查塞尔维亚女性卵巢癌样本中是否存在人乳头瘤病毒（HPV）感染，并将其与疾病的临床病理特征和患者特征相关联。
本研究纳入了 54 例卵巢癌患者。通过盐析法从手术治疗获得的肿瘤组织中提取 DNA。通过 GP5+/GP6+引物扩增 L1 病毒基因的 150bp 片段，通过聚合酶链反应扩增来检测 HPV 感染。通过 DNA 测序进行 HPV 基因分型。
HPV DNA 存在于 54 例卵巢癌中的 4 例（7.4%）中。所有 HPV 阳性肿瘤均含有高危型 HPV16 型。HPV 感染在晚期肿瘤中比局限性肿瘤更为常见。HPV 感染患者的诊断中位年龄为 57 岁，而无 HPV 感染患者的诊断中位年龄为 59 岁。
我们的研究结果表明，HPV 感染可能在卵巢癌发生中发挥有限作用。
