Shokouh Mohammad Reza, Safaei Akbar, Moattari Afagh, Sarvari Jamal
Department of Bacteriology and Virology, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Molecular Pathology, Shiraz University of Medical Sciences, Shiraz, Iran.
Iran J Pathol. 2020 Fall;15(4):292-298. doi: 10.30699/ijp.2020.119681.2306. Epub 2020 Jul 16.
BACKGROUND & OBJECTIVE: Ovarian cancer is one of the most common cancers amongst women. The association of Human papillomavirus (HPV) and Epstein-Barr virus (EBV) with ovarian cancer is inconclusive; therefore, the aims of this study were to evaluate the frequency of HPV and EBV in malignant, borderline, benign and normal ovarian tissues.
In this case-control study, 205 Paraffin-embedded ovarian tissue specimens including 68 malignant, 27 borderline, 65 benign, and 45 normal tissues were included from December 2014 to January 2018 and subjected to DNA extraction. The β-globin gene was amplified using PCR to confirm the quality of the extracted DNA. The genomes of HPV (genotypes 16 and 18) and EBV were identified, using specific primers by PCR.
The mean age of participants was 43.42 ± 15.4 years. The frequency of HPV was statistically significant between malignant versus benign (=0.02) and control groups (=0.002), but not with borderline tumor group (=0.78). Amongst HPV infected samples, 1 (4.5%) and 14 (63.6%) samples were infected with types 16 and 18, respectively. Also 4 (18.2 %) samples were infected with both genotypes. Eleven samples including 7(10.3%) malignant, 1 (3.7%) borderline, 3 (4.6%) benign and none (0%) of normal control groups were infected with EBV, which was statistically different between malignant and the normal control group (=0.03).
The results of our study showed the possible role of high risk HPVs as well as EBV in pathogenesis of ovarian cancer, and further studies are recommended to confirm these findings.
卵巢癌是女性中最常见的癌症之一。人乳头瘤病毒（HPV）和爱泼斯坦-巴尔病毒（EBV）与卵巢癌之间的关联尚无定论；因此，本研究的目的是评估HPV和EBV在恶性、交界性、良性及正常卵巢组织中的感染频率。
在这项病例对照研究中，纳入了205份石蜡包埋的卵巢组织标本，包括68份恶性组织、27份交界性组织、65份良性组织和45份正常组织，标本取自2014年12月至2018年1月，并进行DNA提取。使用聚合酶链反应（PCR）扩增β-珠蛋白基因以确认提取DNA的质量。采用特异性引物通过PCR鉴定HPV（16型和18型）和EBV的基因组。
参与者的平均年龄为43.42±15.4岁。HPV感染频率在恶性组织与良性组织（P=0.02）及对照组（P=0.002）之间具有统计学意义，但与交界性肿瘤组相比无统计学意义（P=0.78）。在HPV感染的样本中，分别有1份（4.5%）和14份（63.6%）样本感染16型和18型。此外，4份（18.2%）样本同时感染了这两种基因型。11份样本感染了EBV，其中包括7份（10.3%）恶性组织、1份（3.7%）交界性组织、3份（4.6%）良性组织，正常对照组无一例感染（0%），恶性组织与正常对照组之间差异具有统计学意义（P=0.03）。
我们的研究结果表明高危型HPV以及EBV在卵巢癌发病机制中可能发挥作用，建议进一步研究以证实这些发现。
