Ben Slimane S, Guidoin R, Mourad W, Hébert J, King M W, Sigot-Luizard M F
Laboratory of Experimental Surgery, Laval University, Quebec City, Canada.
Eur Surg Res. 1988;20(1):12-7. doi: 10.1159/000128735.
One of the techniques used to avoid preclotting a porous textile arterial prosthesis is to coat the graft with a layer of a bioerodible polymer. The efficacy of this treatment is dependent in part on the rate at which the polymer degrades after implantation. The focus of this study was therefore to investigate the rate of in vivo degradation of albumin-coated polyester (Dacron)-knitted vascular prostheses. Two types of cross-linked albumin were included: one using glutaraldehyde, the other using carbodiimide as the cross-linking agent. Radioactively labeled albumin-coated prostheses were implanted in the thoracic aorta and peritoneal cavity of dogs, and their rates of biodegradation in vivo were monitored over a 4-week period. The rate of biodegradation was found to depend upon the site of implantation. It occurred more rapidly in the peritoneal cavity where less than 20% of the albumin coating remained after 4 weeks in vivo in comparison to the approximately 30% in the thoracic aorta. The nature and intensity of the cellular response appeared to be related to the cytotoxic potential of the cross-linking agent. Glutaraldehyde induced an inflammatory response and a delay in healing, whereas carbodiimide caused only a mild tissue reaction.
避免多孔纺织动脉假体预凝血的技术之一是用一层可生物降解的聚合物覆盖移植物。这种治疗的效果部分取决于聚合物植入后降解的速度。因此，本研究的重点是研究白蛋白包被的聚酯（涤纶）编织血管假体的体内降解速率。包括两种类型的交联白蛋白：一种使用戊二醛，另一种使用碳二亚胺作为交联剂。将放射性标记的白蛋白包被假体植入犬的胸主动脉和腹腔，并在4周内监测其体内生物降解速率。发现生物降解速率取决于植入部位。在腹腔中发生得更快，与胸主动脉中约30%相比，体内4周后白蛋白涂层残留不到20%。细胞反应的性质和强度似乎与交联剂的细胞毒性潜力有关。戊二醛诱导炎症反应并延迟愈合，而碳二亚胺仅引起轻微的组织反应。
