Kilari Eswar Kumar, Putta Swathi
a Pharmacology Division, A.U. College of Pharmaceutical Sciences, Andhra University , Visakhapatnam , India.
Cutan Ocul Toxicol. 2017 Mar;36(1):52-59. doi: 10.3109/15569527.2016.1144610. Epub 2016 May 10.
The study was carried out to evaluate the effect of the aqueous fruit pericarp extract of Litchi chinensis (APLC) on parameters which leads to diabetic cataractogenesis and retinopathy in the streptozotocin-induced diabetic rats.
The objective of the study is to evaluate the APLC for in vivo antioxidant activity and its role in inhibiting the polyol pathway and formation of advanced glycation end products (AGEs).
The diabetic animals were treated with L. chinensis for a period of 12 weeks. At the end of 12 weeks, the animals were killed and the biochemical pathways involved in the pathogenesis of cataract such as oxidative stress by protein content, superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH), and polyolpathway by aldose reductase (AR) in lens homogenates, alterations in protein carbonyl content (PCO) and AGEs in both serum and lens the APLC-treated diabetic rats were compared against diabetic control rats. Cataract progression due to hyperglycemia was monitored by slit lamp bio microscope and classified into four stages. Fundoscope test and retinal histopathology were done for assessing retinopathy.
Statistically significant reduction in glucose, and elevation of protein content, SOD, CAT, and GSH levels and decreased levels of AR and PCO in lens homogenate and significant reduction in AGEs serum and lens homogenate were observed. Slit lamp examination, fundoscope, and histopathology showed improvement in retinal changes in APLC-treated rats compared to diabetic control animals.
The treatment with APLC found to delay the progression of diabetic cataractogenesis and retinopathy, which might be due to its antioxidant activity, because of the presence of active phytochemicals in APLC.
本研究旨在评估荔枝果皮水提取物（APLC）对链脲佐菌素诱导的糖尿病大鼠中导致糖尿病性白内障形成和视网膜病变的参数的影响。
本研究的目的是评估APLC的体内抗氧化活性及其在抑制多元醇途径和晚期糖基化终产物（AGEs）形成中的作用。
糖尿病动物用荔枝治疗12周。在12周结束时，处死动物，比较APLC治疗的糖尿病大鼠与糖尿病对照大鼠晶状体匀浆中与白内障发病机制相关的生化途径，如通过蛋白质含量、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、还原型谷胱甘肽（GSH）检测氧化应激，通过醛糖还原酶（AR）检测多元醇途径，血清和晶状体中蛋白质羰基含量（PCO）和AGEs的变化。用裂隙灯生物显微镜监测高血糖导致的白内障进展并分为四个阶段。进行检眼镜检查和视网膜组织病理学检查以评估视网膜病变。
观察到血糖有统计学意义的降低，晶状体匀浆中蛋白质含量、SOD、CAT和GSH水平升高，AR和PCO水平降低，血清和晶状体匀浆中AGEs显著降低。裂隙灯检查、检眼镜检查和组织病理学显示，与糖尿病对照动物相比，APLC治疗的大鼠视网膜变化有所改善。
发现APLC治疗可延缓糖尿病性白内障形成和视网膜病变的进展，这可能归因于其抗氧化活性，因为APLC中存在活性植物化学物质。
