Yuan Wenwen, Chang Huasong, Liu Xinying, Wang Shiqiang, Liu Hui, Xuan Hongzhuan
School of Life Science, Liaocheng University, Liaocheng 252059, China.
Center of Bee Industry on Seed-Breeding and Popularization in Shandong Province, Tai'an, China.
Evid Based Complement Alternat Med. 2019 Jul 7;2019:5789574. doi: 10.1155/2019/5789574. eCollection 2019.
Propolis has been widely used as a dietary supplement for its health benefits, including cardiovascular protective effects. The aim of this study was to investigate the cytoprotective effects of Brazilian green propolis (BP) against oxidized low-density lipoprotein (Ox-LDL) induced human umbilical vein endothelial cells (HUVECs) damage. Our results suggested that treatment with BP rescued Ox-LDL-stimulated HUVECs cell viability losses, which might be associated with its inhibitive effects on the cell apoptosis and autophagy. We also noticed that BP restored Ox-LDL-stimulated HUVECs oxidative stress, by induced antioxidant gene expressions, including Heme oxygenase-1 and its upstream mediator, Nrf2, which were mediated by the activation of the phosphorylation of PI3K/Akt/mTOR. Pretreatment with wortmannin, PI3K/AKT inhibitor, abolished BP induced Nrf2 nuclear translocation and HO-1 level. Our results demonstrated that BP protected HUVECs against oxidative damage partly via PI3K/Akt/mTOR-mediated Nrf/HO-1 pathway, which might be applied into preventing Ox-LDL mediated cardiovascular diseases.
蜂胶因其对健康有益，包括对心血管的保护作用，而被广泛用作膳食补充剂。本研究的目的是探讨巴西绿蜂胶（BP）对氧化低密度脂蛋白（Ox-LDL）诱导的人脐静脉内皮细胞（HUVECs）损伤的细胞保护作用。我们的结果表明，BP处理可挽救Ox-LDL刺激的HUVECs细胞活力损失，这可能与其对细胞凋亡和自噬的抑制作用有关。我们还注意到，BP通过诱导抗氧化基因表达，包括血红素加氧酶-1及其上游介质Nrf2，恢复了Ox-LDL刺激的HUVECs氧化应激，这是由PI3K/Akt/mTOR磷酸化的激活介导的。用渥曼青霉素（PI3K/AKT抑制剂）预处理可消除BP诱导的Nrf2核转位和HO-1水平。我们的结果表明，BP部分通过PI3K/Akt/mTOR介导的Nrf/HO-1途径保护HUVECs免受氧化损伤，这可能应用于预防Ox-LDL介导的心血管疾病。
