Department of Otorhinolaryngology, Medical University of Vienna, Waehringer Guertel 18-20, 1090, Vienna, Austria.
Department of Cranio-Maxillofacial and Oral Surgery, Medical University of Vienna, Waehringer Guertel 18-20, 1090, Vienna, Austria.
J Mater Sci Mater Med. 2018 Apr 9;29(4):45. doi: 10.1007/s10856-018-6051-7.
In the upper aerodigestive tract, biofilm deposits by oropharyngeal microbes can cause failure of medical polymer devices like voice prostheses. Previous studies on testing of inhibitive strategies still lack of comparability due to varying study protocols concerning growth media, microbial species and growth conditions. Goal of the study was therefore to test cultivation of a mixed biofilm of isolated oropharyngeal microbes under in vitro growth conditions using mixtures of common growth media. Mixtures of yeast peptone dextrose medium (YPD), fetal bovine serum (FBS), RPMI 1640, Yeast nitrogen base medium (YNB) and brain heart infusion (BHI) were tested to grow mixed biofilm deposits of Candida albicans, Candida tropicalis, Staphylococcus aureus, Streptococcus epidermidis, Rothia dentocariosa and Lactobacillus gasseri on medical grade silicone. Periodic assessment of living biofilm was performed over 22 days by a digital microscope and the cultivated biofilm structures were analyzed by scanning electron microscopy after completion of the study. Mixtures of BHI, YPD and FBS improved microscopic growth of multispecies biofilm deposits over time, while addition of RPMI and YNB resulted in reduction of visible biofilm deposit sizes. A mixtures of FBS 30% + YPD 70% and BHI 30% + YPD 70% showed enhanced support of permanent surface growth on silicone. Growth kinetics of in vitro multispecies biofilms can be manipulated by using mixtures of common growth media. Using mixtures of growth media can improve growth of longterm multispecies oropharyngeal biofilm models used for in vitro testing of antibiofilm materials or coatings for voice prostheses.
在上呼吸道，口咽微生物的生物膜沉积物可导致医用聚合物器械（如人工发声器）失效。由于涉及不同的生长介质、微生物种类和生长条件，以前关于抑制策略的测试研究仍然缺乏可比性。因此，本研究的目的是使用常见的生长培养基混合物，在体外生长条件下测试分离的口咽微生物混合生物膜的培养。研究测试了酵母肽葡萄糖培养基 (YPD)、胎牛血清 (FBS)、RPMI 1640、酵母氮基培养基 (YNB) 和脑心浸液 (BHI) 的混合物，以在医用级硅酮上生长白色念珠菌、热带念珠菌、金黄色葡萄球菌、表皮葡萄球菌、罗特氏菌和嗜酸乳杆菌的混合生物膜沉积物。通过数字显微镜定期评估活生物膜，在研究完成后，通过扫描电子显微镜分析培养的生物膜结构。BHI、YPD 和 FBS 的混合物随着时间的推移改善了多物种生物膜沉积物的微观生长，而 RPMI 和 YNB 的添加导致可见生物膜沉积物尺寸减小。FBS 30%+YPD 70%和 BHI 30%+YPD 70%的混合物显示出对硅酮上永久性表面生长的更好支持。通过使用常见生长培养基的混合物，可以操纵体外多物种生物膜的生长动力学。使用生长培养基的混合物可以改善用于人工发声器的抗生物膜材料或涂层的体外测试的长期多物种口咽生物膜模型的生长。
