Food Research Division, Bureau of Chemical Safety, Food Directorate-HPFB, Health Canada , Ottawa , ON , Canada.
Analytical Laboratory, CER Groupe , Marloie , Belgium.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2019 Oct;36(10):1567-1573. doi: 10.1080/19440049.2019.1640895. Epub 2019 Jul 30.
A sensitive competitive indirect enzyme-linked immunosorbent assay (ciELISA) was developed for the detection and quantification of citrinin (CIT) in grain-based food samples. The limit of quantification (IC) of the established method was 0.10 ± 0.02 ng mL, with the limit of detection (IC) being 0.04 ± 0.007 ng mL in wheat and corn flour matrices with a coefficient of variation (CV) less than 20%. The assay was very specific to CIT and showed no cross-reactivity with other mycotoxins (OTA, T-2 toxin, HT-2 toxin, DON, patulin and zearalenone). In spiked wheat and corn flours, the recoveries ranged from 86.6% to 115.6% with CVs of less than 20%. The effectiveness of this method was verified by participating in a proficiency test (PT) from the Food Analysis Performance Assessment Scheme (FAPAS) 17181 corn flour. A successful z-score (-0.6) for this PT sample showed that the present method is comparable to the instrumental methods used by other laboratories in the PT testing scheme. A small survey of grain-based foods was conducted using this method and CIT was detected in 43% of the samples up to a concentration of 17.7 ng g. This method is suitable for sensitive and rapid quantitation of citrinin in wheat and corn matrices.
建立了一种用于检测和定量谷物食品中桔青霉素（CIT）的灵敏竞争性间接酶联免疫吸附测定法（ciELISA）。该方法的定量下限（IC）为 0.10±0.02ng mL，在小麦和玉米粉基质中的检测限（IC）为 0.04±0.007ng mL，变异系数（CV）小于 20%。该测定法对 CIT 具有很高的特异性，与其他霉菌毒素（OTA、T-2 毒素、HT-2 毒素、DON、展青霉素和玉米赤霉烯酮）无交叉反应。在添加的小麦和玉米粉中，回收率在 86.6%至 115.6%之间，CV 小于 20%。该方法通过参加食品分析性能评估计划（FAPAS）17181 玉米粉的能力验证（PT）得到了验证。该 PT 样品的成功 z 值（-0.6）表明，该方法可与 PT 测试方案中其他实验室使用的仪器方法相媲美。使用该方法对谷物食品进行了小范围调查，在高达 17.7ng g 的浓度下，在 43%的样品中检测到 CIT。该方法适用于小麦和玉米基质中 CIT 的灵敏快速定量。
