Graduate Institute of Medicine, Chung Shan Medical University, Taichung 402, Taiwan.
Department of Biomedical Sciences, Chung Shan Medical University, Taichung 402, Taiwan.
Toxins (Basel). 2018 Sep 2;10(9):354. doi: 10.3390/toxins10090354.
Antibodies against citrinin (CTN) were generated from rabbits, which were injected with CTN-keyhole limpet hemocyanin (KLH). This work involved the development of a sensitive competitive direct enzyme-linked immunosorbent assay (cdELISA) and a rapid gold nanoparticle immunochromatographic strip (immunostrip) method for analyzing CTN in -fermented food. CTN at a concentration of 5.0 ng/mL caused 50% inhibition (IC) of CTN-horseradish peroxidase (CTN-HRP) binding to the antibodies in the cdELISA. The capable on-site detection of CTN was accomplished by a rapid antibody-gold nanoparticle immunostrip with a detection limit of 20 ng/mL and that was completed within 15 min. A close inspection of 19 -fermented foods by cdELISA confirmed that 14 were contaminated with citrinin at levels from 28.6⁻9454 ng/g. Further analysis with the immunostrip is consistent with those results obtained using cdELISA. Both means are sensitive enough for the rapid examination of CTN in -fermented food products.
从兔子中产生了针对桔青霉素（CTN）的抗体，这些兔子被注射了 CTN-血蓝蛋白（KLH）。本工作开发了一种灵敏的竞争直接酶联免疫吸附测定（cdELISA）和一种快速的金纳米颗粒免疫层析条（免疫层析条）方法，用于分析发酵食品中的 CTN。在 cdELISA 中，CTN 的浓度为 5.0ng/mL 时，会引起 CTN-辣根过氧化物酶（CTN-HRP）与抗体结合的 50%抑制（IC）。通过快速的抗体-金纳米颗粒免疫层析条，可以在 15 分钟内完成对 CTN 的现场检测，检测限为 20ng/mL。通过 cdELISA 对 19 种发酵食品进行的仔细检查证实，其中 14 种食品受到 CTN 的污染，污染水平为 28.6⁻9454ng/g。使用免疫层析条进行的进一步分析与 cdELISA 获得的结果一致。这两种方法都足够灵敏，可以快速检测发酵食品中的 CTN。
