建立一种灵敏的酶联免疫吸附测定法和快速金纳米免疫层析试纸条用于发酵食品中桔青霉素的检测。
Development of a Sensitive Enzyme-Linked Immunosorbent Assay and Rapid Gold Nanoparticle Immunochromatographic Strip for Detecting Citrinin in Fermented Food.
机构信息
Graduate Institute of Medicine, Chung Shan Medical University, Taichung 402, Taiwan.
Department of Biomedical Sciences, Chung Shan Medical University, Taichung 402, Taiwan.
出版信息
Toxins (Basel). 2018 Sep 2;10(9):354. doi: 10.3390/toxins10090354.
Antibodies against citrinin (CTN) were generated from rabbits, which were injected with CTN-keyhole limpet hemocyanin (KLH). This work involved the development of a sensitive competitive direct enzyme-linked immunosorbent assay (cdELISA) and a rapid gold nanoparticle immunochromatographic strip (immunostrip) method for analyzing CTN in -fermented food. CTN at a concentration of 5.0 ng/mL caused 50% inhibition (IC) of CTN-horseradish peroxidase (CTN-HRP) binding to the antibodies in the cdELISA. The capable on-site detection of CTN was accomplished by a rapid antibody-gold nanoparticle immunostrip with a detection limit of 20 ng/mL and that was completed within 15 min. A close inspection of 19 -fermented foods by cdELISA confirmed that 14 were contaminated with citrinin at levels from 28.6⁻9454 ng/g. Further analysis with the immunostrip is consistent with those results obtained using cdELISA. Both means are sensitive enough for the rapid examination of CTN in -fermented food products.
从兔子中产生了针对桔青霉素（CTN）的抗体，这些兔子被注射了 CTN-血蓝蛋白（KLH）。本工作开发了一种灵敏的竞争直接酶联免疫吸附测定（cdELISA）和一种快速的金纳米颗粒免疫层析条（免疫层析条）方法，用于分析发酵食品中的 CTN。在 cdELISA 中，CTN 的浓度为 5.0ng/mL 时，会引起 CTN-辣根过氧化物酶（CTN-HRP）与抗体结合的 50%抑制（IC）。通过快速的抗体-金纳米颗粒免疫层析条，可以在 15 分钟内完成对 CTN 的现场检测，检测限为 20ng/mL。通过 cdELISA 对 19 种发酵食品进行的仔细检查证实，其中 14 种食品受到 CTN 的污染，污染水平为 28.6⁻9454ng/g。使用免疫层析条进行的进一步分析与 cdELISA 获得的结果一致。这两种方法都足够灵敏，可以快速检测发酵食品中的 CTN。
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