Department of Biosciences, COMSATS University (CUI), Tarlai Kalan, Chak Shahzad, Islamabad, Pakistan.
KU Leuven-University of Leuven, Department of Microbiology and Immunology, Rega Institute for Medical Research, Laboratory of Viral Metagenomics, Leuven, Belgium.
PLoS One. 2019 Jul 30;14(7):e0220387. doi: 10.1371/journal.pone.0220387. eCollection 2019.
Group A rotaviruses (RVA) are one of the major causes of acute gastroenteritis (AGE) in young children worldwide. Owing to lack of proper surveillance programs and health facilities, developing countries of Asia and Africa carry a disproportionately heavy share of the RVA disease burden. The aim of this hospital-based study was to investigate the circulation of RVA genotypes in Rawalpindi and Islamabad, Pakistan in 2015 and 2016, prior to the implementation of RVA vaccine. 639 faecal samples collected from children under 10 years of age hospitalized with AGE were tested for RVA antigen by ELISA. Among 171 ELISA positive samples, 143 were successfully screened for RT-PCR and sequencing. The prevalence of RVA was found to be 26.8% with the highest frequency (34.9%) found among children of age group 6-11 months. The most predominant circulating genotypes were G3P[8] (22.4%) followed by G12P[6] (20.3%), G2P[4] (12.6%), G1P[8] (11.9%), G9P[6] (11.9%), G3P[4] (9.1%), G1P[6] (4.2%), G9P[8] (4.2%), and G3P[6] (0.7%). A single mixed genotype G1G3P[8] was also detected. The findings of this study provide baseline data, that will help to assess if future vaccination campaigns using currently available RVA vaccine will reduce RVA disease burden and instigate evolutionary changes in the overall RVA biology. The high prevalence of RVA infections in Pakistan require to improve and strengthen the surveillance and monitoring system for RVA. This will provide useful information for health authorities in planning public health care strategies to mitigate the disease burden caused by RVA.
A 组轮状病毒（RVA）是全球导致婴幼儿急性肠胃炎（AGE）的主要原因之一。由于缺乏适当的监测计划和卫生设施，亚洲和非洲的发展中国家承担着不成比例的 RVA 疾病负担。本基于医院的研究旨在调查 2015 年和 2016 年巴基斯坦拉瓦尔品第和伊斯兰堡 RVA 基因型的流行情况，此时 RVA 疫苗尚未实施。从因 AGE 住院的 10 岁以下儿童中收集了 639 份粪便样本，通过 ELISA 检测 RVA 抗原。在 171 份 ELISA 阳性样本中，有 143 份成功进行了 RT-PCR 和测序筛查。RVA 的流行率为 26.8%，年龄组为 6-11 个月的儿童中发现的频率最高（34.9%）。最主要的循环基因型是 G3P[8]（22.4%），其次是 G12P[6]（20.3%）、G2P[4]（12.6%）、G1P[8]（11.9%）、G9P[6]（11.9%）、G3P[4]（9.1%）、G1P[6]（4.2%）、G9P[8]（4.2%）和 G3P[6]（0.7%）。还检测到一种单一的混合基因型 G1G3P[8]。本研究的结果提供了基线数据，这将有助于评估当前可用的 RVA 疫苗未来的疫苗接种活动是否会降低 RVA 疾病负担并引发 RVA 生物学的进化变化。RVA 感染在巴基斯坦的高流行率需要改善和加强 RVA 的监测和监测系统。这将为卫生当局规划减轻 RVA 引起的疾病负担的公共卫生保健策略提供有用的信息。
