Department of Microbiology, University of Jhang, Jhang, Pakistan.
Department of Microbiology, Quaid-i-Azam University, Islamabad, Pakistan.
Biomed Res Int. 2022 Sep 22;2022:6972624. doi: 10.1155/2022/6972624. eCollection 2022.
Celiac disease (CD) is a chronic inflammatory disorder in the intestinal tract as a response to the use of gluten in genetically predisposed individuals. It is a worldwide problem, with a high prevalence rate in North America. This is a descriptive cross-sectional study involving 1090 samples collected from different hospitals of Rawalpindi and Islamabad, Pakistan, from January 2019 to December 2019. In this study, 1090 blood samples screened for seroprevalence of anti-tTG antibodies in CD symptomatic patients via ELISA (enzyme-linked immunosorbent assay). 1090 fecal samples from the same CD patients were collected and tested for the presence of rotavirus (RV) via ELISA and RT-PCR. Of the 1090 patients tested for seroprevalence of anti-tTG antibodies, 112/1090 (10.3%) were found to be positive. Of the 112 anti-tTG-positive patients, 78/112 (70%) were positive for RV via ELISA and 74/112 (66.1%) were RV positive via RT-PCR. A statistically significant association was reported between rotavirus infection and celiac disease (˂0.05). Anti-tTG antibodies were higher in age group 6 (12-18 years) patients (18.4%) and at minimum in age group 3 (1-3 years) patients (4.8%). However, there was a statistically insignificant relationship between group age and CD prevalence ( > 0.05). The highest CD prevalence was noted during winter season (19.6%) and the lowest (3.0%) during fall/autumn. Our study findings demonstrate that Pakistan has a high prevalence of CD compared to other studies. Further studies in the fields of environmental risk factors and treatment with more advanced serological and histopathological studies are needed in the future.
乳糜泻（CD）是一种肠道慢性炎症性疾病，是遗传易感性个体对麸质使用的反应。它是一个全球性问题，在北美地区发病率较高。这是一项描述性的横断面研究，涉及 2019 年 1 月至 2019 年 12 月从巴基斯坦拉瓦尔品第和伊斯兰堡的不同医院收集的 1090 个样本。在这项研究中，通过 ELISA（酶联免疫吸附试验）对 1090 例 CD 症状患者的血清抗 tTG 抗体进行了血清流行率筛查。从同一 CD 患者中收集了 1090 个粪便样本，并通过 ELISA 和 RT-PCR 检测轮状病毒（RV）的存在。在对 1090 例抗 tTG 抗体血清流行率进行检测的患者中，发现 112/1090（10.3%）呈阳性。在 112 例抗 tTG 阳性患者中，78/112（70%）通过 ELISA 检测 RV 阳性，74/112（66.1%）通过 RT-PCR 检测 RV 阳性。轮状病毒感染与乳糜泻之间存在统计学显著关联（˂0.05）。年龄组 6（12-18 岁）患者的抗 tTG 抗体（18.4%）较高，年龄组 3（1-3 岁）患者的抗 tTG 抗体（4.8%）最低。然而，组年龄与 CD 患病率之间无统计学意义关系（>0.05）。冬季 CD 患病率最高（19.6%），秋季最低（3.0%）。我们的研究结果表明，与其他研究相比，巴基斯坦的 CD 患病率较高。未来需要在环境危险因素和更先进的血清学和组织病理学研究领域开展进一步的研究。
