Centro de Investigación Científica y de Educación Superior de Ensenada Baja California, México, CICESE, Ensenada Baja California, México.
PLoS One. 2019 Jul 30;14(7):e0220385. doi: 10.1371/journal.pone.0220385. eCollection 2019.
Whole-genome sequences of Vibrio vulnificus clinical genotype (C-genotype) from the CICESE Culture Collection, isolated from oysters, were compared with reference sequences of CMCP6 and YJ016 V. vulnificus C-genotype strains of clinical origin. The RAST web server estimated the whole genome to be ~4.8 Mb in CICESE strain 316 and ~4.7 Mb in CICESE strain 325. No plasmids were detected in the CICESE strains. Based on a phylogenetic tree that was constructed with the whole-genome results, we observed high similarity between the reference sequences and oyster C-genotype isolates and a sharp contrast with environmental genotype (E-genotype) reference sequences, indicating that the differences between the C- and E-genotypes do not necessarily correspond to their isolation origin. The CICESE strains share 3488 genes (63.2%) with the YJ016 strain and 3500 genes (63.9%) with the CMCP6 strain. A total of 237 pathogenicity associated genes were selected from reference clinical strains, where-92 genes were from CMCP6, 126 genes from YJ016, and 19 from MO6-24/O; the presence or absence of these genes was recorded for the CICESE strains. Of the 92 genes that were selected for CMCP6, 67 were present in both CICESE strains, as were as 86 of the 126 YJ016 genes and 13 of the 19 MO6-24/O genes. The detection of elements that are related to virulence in CICESE strains-such as the RTX gene cluster, vvhA and vvpE, the type IV pili cluster, the XII genomic island, and the viuB genes, suggests that environmental isolates with the C-genotype, have significant potential for infection.
从 CICESE 培养物收集分离的、具有临床基因型 (C 基因型) 的创伤弧菌全基因组序列与来自临床的 CMCP6 和 YJ016 V. vulnificus C 基因型菌株的参考序列进行了比较。RAST 网络服务器估计 CICESE 菌株 316 的全基因组约为 4.8 Mb，CICESE 菌株 325 的全基因组约为 4.7 Mb。在 CICESE 菌株中未检测到质粒。根据全基因组结果构建的系统发育树表明，参考序列与牡蛎 C 基因型分离株高度相似，与环境基因型 (E 基因型) 参考序列形成鲜明对比，表明 C 基因型和 E 基因型之间的差异不一定与其分离来源相对应。CICESE 株与 YJ016 株共享 3488 个基因（63.2%），与 CMCP6 株共享 3500 个基因（63.9%）。从参考临床菌株中选择了总共 237 个与致病性相关的基因，其中 92 个基因来自 CMCP6，126 个基因来自 YJ016，19 个基因来自 MO6-24/O；记录了这些基因在 CICESE 株中的存在或缺失情况。在选择用于 CMCP6 的 92 个基因中，有 67 个基因存在于两个 CICESE 株中，126 个 YJ016 基因中有 86 个基因存在，19 个 MO6-24/O 基因中有 13 个基因存在。在 CICESE 株中检测到与毒力相关的元素，如 RTX 基因簇、vvhA 和 vvpE、IV 型菌毛簇、XII 基因组岛和 viuB 基因，表明具有 C 基因型的环境分离株具有显著的感染潜力。
