Department of Chemical Engineering, North Tehran Branch, Islamic Azad University, PO Box 1951153311, Tehran, Iran.
Department of Chemical Engineering, North Tehran Branch, Islamic Azad University, PO Box 1951153311, Tehran, Iran.
Colloids Surf B Biointerfaces. 2019 Oct 1;182:110368. doi: 10.1016/j.colsurfb.2019.110368. Epub 2019 Jul 17.
In an ideal delivery system, carrier nanoparticles are used as a promising alternative with minimized adverse effects to treat a variety of diseases. The purpose of this study was to create a targeted delivery system for doxorubicin hydrochloride (DOX-HCl), using FeO-L@HSA-β-cyclodextrin (β-CD)/Allyl amine nanoparticles. In this study, magnetite nanoparticles (FeO) were produced by co-precipitation, while albumin nanoparticles (HSA) were produced by the desolvation method. The properties of the nanoparticles were studied by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and vibrating sample magnetometer (VSM). To evaluate the loading of the drug on the synthesized nanoparticles, ultraviolet-visible spectrophotometry (UV-Vis) and atomic force microscopy (AFM) were used. DOX-HCl loading was studied by two experiments: an optimization method (OM) and a one-factor-at-a-time method (OFATM). Response surface methodology (RSM) was utilized to optimize the parameters. The optimal conditions for drug loading of nanoparticles in OM and OFATM methods were 81.46% and 77%, respectively. The release of DOX-HCl drug from the synthesized nanoparticles at a temperature of 37 °C and specific time in pH 5.3 and pH 7.4 was 83.35% and 38.39%, respectively. To examine the cytotoxicity of nanoparticles with drugs, the MTT assay was performed using MCF-7 cancer cells. Finally, cell uptake was tested using inductively coupled plasma-mass spectrometry (ICP-MS).
在理想的给药系统中，载体纳米粒子作为一种有前途的替代品被广泛应用，以最小化不良反应来治疗各种疾病。本研究旨在通过 FeO-L@HSA-β-环糊精（β-CD）/烯丙胺纳米粒构建盐酸多柔比星（DOX-HCl）的靶向给药系统。在本研究中，通过共沉淀法制备了磁铁矿纳米粒子（FeO），通过去溶剂法制备了白蛋白纳米粒子（HSA）。通过扫描电子显微镜（SEM）、傅里叶变换红外光谱（FT-IR）、X 射线衍射（XRD）和振动样品磁强计（VSM）研究了纳米粒子的性质。为了评估药物在合成纳米粒子上的负载量，使用了紫外可见分光光度法（UV-Vis）和原子力显微镜（AFM）。通过两种实验（优化方法（OM）和单因素法（OFATM））研究了 DOX-HCl 的负载量。响应面法（RSM）用于优化参数。在 OM 和 OFATM 方法中，纳米粒子药物载量的最佳条件分别为 81.46%和 77%。在 37°C 和特定时间的 pH 5.3 和 pH 7.4 条件下，从合成的纳米粒子中释放 DOX-HCl 药物的比例分别为 83.35%和 38.39%。为了研究载药纳米粒子的细胞毒性，使用 MCF-7 癌细胞进行了 MTT 检测。最后，使用电感耦合等离子体质谱法（ICP-MS）测试了细胞摄取。
