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甘蓝提取物(白花甘蓝和西兰花)的抗菌潜力及其与强力霉素相比对革兰氏阳性菌和革兰氏阴性菌的耐药性

Antimicrobial Potential of Brassica oleracea Extracts (White and Broccoli) and Their Resistance Compared to Doxycycline Against Gram-Positive and Gram-Negative Bacteria.

作者信息

Rahmani Meisam, Esmaeili Akbar, Taherkhani Mahboubeh

机构信息

Department of Basic Science, Central Tehran Branch Islamic Azad University Tehran Iran.

Department of Chemical Engineering, North Tehran Branch Islamic Azad University Tehran Iran.

出版信息

Food Sci Nutr. 2024 Jul 28;12(10):7492-7503. doi: 10.1002/fsn3.4345. eCollection 2024 Oct.

Abstract

L is low in carbohydrates and fiber, making it ideal for low-carb diets. But they are rich in vitamins, minerals, and other essential nutrients. The present article investigated the antimicrobial effect of extract (white and broccoli) (BOE-WB) on Gram-positive and Gram-negative strains and its resistance to doxycycline. BOE-WB was used as the soaking method. It was analyzed by gas chromatography-mass spectroscopy (GC-MS), high-performance liquid chromatography (HPLC), and 2,2-Diphenyl-1-picrylhydrazyl (DPPH) methods. The samples were prepared with three concentrations. It used Mueller Hinton Agar culture medium of fresh bacteria. It was incubated for 24 h in a 37°C incubator. It identified 33 compounds. For BOE-W, 77% of the compounds are oxygenated, while for BOE-B, this percentage is 79%. For BOE-B, 79% had oxygenated compounds. BOE-WB has significant antibacterial effects on and . The lethal impact of BOE-WB on strains is very close to that of doxycycline, and it can be introduced as a new antimicrobial drug to the medical world. The research shows that the percentage of oxygenated compounds in foods containing BOE-B is much higher than that of BOE-W. It has a significant impact on the antioxidant effect. Foods containing BOE-W have a high percentage of azo compounds and sulfur. One of the benefits of sulfur in the body is disinfecting the blood. In addition, sulfur increases the body's resistance to bacteria.

摘要

L的碳水化合物和纤维含量低,使其成为低碳水化合物饮食的理想选择。但它们富含维生素、矿物质和其他必需营养素。本文研究了提取物(白色和西兰花)(BOE-WB)对革兰氏阳性菌和革兰氏阴性菌的抗菌作用及其对强力霉素的耐药性。采用浸泡法使用BOE-WB。通过气相色谱-质谱联用(GC-MS)、高效液相色谱(HPLC)和2,2-二苯基-1-苦基肼(DPPH)方法进行分析。样品制备了三种浓度。使用新鲜细菌的穆勒-欣顿琼脂培养基。在37°C培养箱中孵育24小时。鉴定出33种化合物。对于BOE-W,77%的化合物是氧化型的,而对于BOE-B,这一比例为79%。对于BOE-B,79%含有氧化型化合物。BOE-WB对[具体菌株1]和[具体菌株2]有显著的抗菌作用。BOE-WB对菌株的致死作用与强力霉素非常接近,可作为一种新型抗菌药物引入医学领域。研究表明,含BOE-B的食物中氧化型化合物的比例远高于BOE-W。这对抗氧化作用有显著影响。含BOE-W的食物中偶氮化合物和硫的比例较高。体内硫的好处之一是对血液进行消毒。此外,硫可增强人体对细菌的抵抗力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0773/11521745/33c0870f93d9/FSN3-12-7492-g005.jpg

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