Research Laboratory of Dendrimers and Nanopolymers, Faculty of Chemistry, University of Tabriz, P.O. Box 51666, Tabriz, Iran.
Research Laboratory of Dendrimers and Nanopolymers, Faculty of Chemistry, University of Tabriz, P.O. Box 51666, Tabriz, Iran; Research Center for Pharmaceutical Nanotechnology (RCPN), Tabriz University of Medical Science, Tabriz, Iran.
Int J Biol Macromol. 2020 Aug 1;156:648-659. doi: 10.1016/j.ijbiomac.2020.04.037. Epub 2020 Apr 11.
In the present study, a novel magnetic carbon modified with 3-aminopropyltrimethoxysilane (APTMS) using maltose disaccharide molecule as a green capping agent, and a third-generation triazine dendrimer (FeO@C@TD-G3) was then covalently attached to their surface. Eventually, FeO@C@TD-G3 was reacted with graphene quantum dots (GQDs) for the preparation of final FeO@C@TDGQDs microspheres. The obtained compound was successfully applied as a novel magnetic carrier for the Doxorubicin (DOX) drug delivery. Various techniques such as Fourier transform infrared (FT-IR), X-ray diffraction (XRD), Zeta potential, Ultraviolet-visible (UV-vis), Atomic force microscopy (AFM), Scanning electron microscopy (SEM), Energy-dispersive X-ray (EDX), Brunauer-Emmett-Teller (BET), Vibration sample magnetometer (VSM), and Fluorescence and Photoluminescence (PL) analysis were used to approve the synthesis of microspheres. Drug release studies carried out at different pHs (pH 5, 6.8 and 7.4) and the cytotoxic assay was evaluated for DOX-loaded FeO@C@TDGQDs against Human lung cancer cell lines (A549). The drug release assay showed that the amount of the DOX release from dendrimer was noticeably pH depended. In-vitro cytotoxicity test results indicated that DOX loaded FeO@C@TDGQDs was non-toxic on the A549 cell. The obtained results demonstrated that FeO@C@TDGQDs microspheres can be used as a new safe and efficient vehicle for the delivery of different cancer drugs.
在本研究中，使用麦芽糖二糖分子作为绿色封端剂，通过 3-氨丙基三甲氧基硅烷 (APTMS) 对一种新型磁性碳进行修饰，并将第三代三嗪树枝状大分子 (FeO@C@TD-G3) 共价连接到其表面。最后，将 FeO@C@TD-G3 与石墨烯量子点 (GQDs) 反应，制备最终的 FeO@C@TDGQDs 微球。所得化合物成功用作阿霉素 (DOX) 药物递送的新型磁性载体。采用傅里叶变换红外 (FT-IR)、X 射线衍射 (XRD)、Zeta 电位、紫外-可见 (UV-vis)、原子力显微镜 (AFM)、扫描电子显微镜 (SEM)、能谱仪 (EDX) 、Brunauer-Emmett-Teller (BET)、振动样品磁强计 (VSM) 和荧光和光致发光 (PL) 分析等多种技术来证实微球的合成。在不同 pH 值 (pH 5、6.8 和 7.4) 下进行药物释放研究，并评估载 DOX 的 FeO@C@TDGQDs 对人肺癌细胞系 (A549) 的细胞毒性测定。药物释放实验表明，树枝状大分子中 DOX 的释放量明显依赖于 pH 值。体外细胞毒性试验结果表明，载 DOX 的 FeO@C@TDGQDs 对 A549 细胞无毒。所得结果表明，FeO@C@TDGQDs 微球可用作递送不同癌症药物的新型安全有效的载体。
