2000-2016 年巴西食品生产链中传播的高优先级沙门氏菌血清型的基因组特征。
Genomic Features of High-Priority Salmonella enterica Serovars Circulating in the Food Production Chain, Brazil, 2000-2016.
机构信息
Department of Food and Experimental Nutrition, Faculty of Pharmaceutical Sciences, Food Research Center, University of São Paulo, São Paulo, Brazil.
Department of Population Health and Pathobiology, North Carolina State University, College of Veterinary Medicine, Raleigh, North Carolina, USA.
出版信息
Sci Rep. 2019 Jul 30;9(1):11058. doi: 10.1038/s41598-019-45838-0.
Multidrug-resistant (MDR) Salmonella enterica has been deemed a high-priority pathogen by the World Health Organization. Two hundred and sixty-four Salmonella enterica isolates recovered over a 16-year period (2000 to 2016) from the poultry and swine production chains, in Brazil, were investigated by whole-genome sequencing (WGS). Most international lineages belonging to 28 serovars, including, S. enterica serovars S. Schwarzengrund ST96, S. Typhimurium ST19, S. Minnesota ST548, S. Infantis ST32, S. Heidelberg ST15, S. Newport ST45, S. Brandenburg ST65 and S. Kentucky ST198 displayed MDR and virulent genetic backgrounds. In this regard, resistome analysis revealed presence of qnrE1 (identified for the first time in S. Typhimurium from food chain), qnrB19, qnrS1, bla, bla and bla genes, as well as gyrA mutations; whereas ColpVC, IncHI2A, IncHI2, IncFIA, Incl1, IncA/C2, IncR, IncX1 and po111 plasmids were detected. In addition, phylogenetic analysis revealed multiple independent lineages such as S. enterica serovars S. Infantis, S. Schwarzengrund, S. Minnesota, S. Kentucky and S. Brandenburg. In brief, ocurrence and persistence of international lineages of S. enterica serovars in food production chain is supported by conserved genomes and wide virulome and resistome.
世界卫生组织已将多药耐药（MDR）沙门氏菌视为高优先级病原体。巴西在 16 年间（2000 年至 2016 年）从家禽和养猪生产链中回收了 264 株沙门氏菌分离株，通过全基因组测序（WGS）对其进行了研究。属于 28 个血清型的大多数国际谱系，包括 S. enterica 血清型 S. Schwarzengrund ST96、S. Typhimurium ST19、S. Minnesota ST548、S. Infantis ST32、S. Heidelberg ST15、S. Newport ST45、S. Brandenburg ST65 和 S. Kentucky ST198，表现出 MDR 和毒力遗传背景。在这方面，耐药组分析显示存在 qnrE1（首次在食物链中的 S. Typhimurium 中发现）、qnrB19、qnrS1、bla、bla 和 bla 基因，以及 gyrA 突变；而 ColpVC、IncHI2A、IncHI2、IncFIA、Incl1、IncA/C2、IncR、IncX1 和 po111 质粒被检测到。此外，系统发育分析显示存在多个独立的谱系，如 S. enterica 血清型 S. Infantis、S. Schwarzengrund、S. Minnesota、S. Kentucky 和 S. Brandenburg。简而言之，食品生产链中 S. enterica 血清型的国际谱系的发生和持续存在是由保守的基因组和广泛的毒力组和耐药组支持的。
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