Instituto de Investigação e Inovação em Saúde, U.Porto, Porto, Portugal.
Instituto de Engenharia Biomédica, U.Porto, Porto, Portugal.
Sci Rep. 2019 Jul 30;9(1):11050. doi: 10.1038/s41598-019-47491-z.
Nano-hydroxyapatite has been used as an oral care ingredient, being incorporated in several products for the treatment of dental hypersensitivity and enamel remineralisation. Despite its promising results, regulatory and safety concerns have been discussed and questioned by the European Scientific Committee on Consumer Safety (SCCS) regarding the usage of hydroxyapatite nanoparticles in oral care products. In this work, a commercially available nano-hydroxyapatite was characterized and its cytocompatibility towards human gingival fibroblasts was evaluated, as well as its irritation potential using the in vitro HET-CAM assay. All the conditions chosen in this study tried to simulate the tooth brushing procedure and the hydroxyapatite nanoparticles levels normally incorporated in oral care products. The commercial hydroxyapatite nanoparticles used in this study exhibited a rod-like morphology and the expected chemical and phase composition. The set of in vitro cytotoxicity parameters accessed showed that these nanoparticles are highly cytocompatible towards human gingival fibroblasts. Additionally, these nanoparticles did not possess any irritation potential on HET-CAM assay. This study clarifies the issues raised by SCCS and it concludes that this specific nano-hydroxyapatite is cytocompatible, as these nanoparticles did not alter the normal behaviour of the cells. Therefore, they are safe to be used in oral care products.
纳米羟基磷灰石已被用作口腔护理成分，被纳入几种治疗牙本质过敏和牙釉质再矿化的产品中。尽管其结果令人鼓舞，但欧洲消费者安全科学委员会（SCCS）对羟基磷灰石纳米颗粒在口腔护理产品中的使用的监管和安全性问题进行了讨论和质疑。在这项工作中，对一种市售的纳米羟基磷灰石进行了表征，并评估了其对人牙龈成纤维细胞的细胞相容性，以及使用体外 HET-CAM 测定法评估其刺激性潜力。本研究中选择的所有条件都试图模拟刷牙过程和口腔护理产品中通常添加的羟基磷灰石纳米颗粒水平。本研究中使用的商业纳米羟基磷灰石表现出棒状形态以及预期的化学和相组成。评估的一组体外细胞毒性参数表明，这些纳米颗粒对人牙龈成纤维细胞具有高度的细胞相容性。此外，这些纳米颗粒在 HET-CAM 测定中没有任何刺激性潜力。本研究澄清了 SCCS 提出的问题，并得出结论，这种特定的纳米羟基磷灰石具有细胞相容性，因为这些纳米颗粒没有改变细胞的正常行为。因此，它们可安全用于口腔护理产品。
