Institute for Agro-Environmental Sciences, NARO, 3-1-3 Kannondai, Tsukuba, 305-8604, Japan.
School of Human Science and Environment, Hyogo University, 1-1-12 Shinzaike-honcho, Himeji, 607-0092, Japan.
J Plant Res. 2019 Sep;132(5):705-718. doi: 10.1007/s10265-019-01127-5. Epub 2019 Jul 30.
Direct measurements of ecophysiological processes such as leaf photosynthesis are often hampered due to the excessive time required for gas-exchange measurements and the limited availability of multiple gas analyzers. Although recent advancements in commercially available instruments have improved the ability to take measurements more conveniently, the amount of time required for each plant sample to acclimate to chamber conditions has not been sufficiently reduced. Here we describe a system of multiple gas-exchange chambers coupled with a laser spectrometer that employs tunable diode laser absorption spectroscopy (TDLAS) to measure leaf photosynthesis, stomatal conductance, and mesophyll conductance. Using four gas-exchange chambers minimizes the time loss associated with acclimation for each leaf sample. System operation is semiautomatic, and leaf temperature, humidity, and CO concentration can be regulated and monitored remotely by a computer system. The preliminary results with rice leaf samples demonstrated that the system is capable of high-throughput measurements, which is necessary to obtain better representativeness of the ecophysiological characteristics of plant samples.
直接测量叶片光合作用等生理生态过程往往受到气体交换测量所需时间过长和多气体分析仪数量有限的阻碍。尽管商业上可用仪器的最新进展提高了更方便地进行测量的能力，但每个植物样本适应腔室条件所需的时间并没有得到充分减少。在这里，我们描述了一种由多个气体交换室组成的系统，该系统与激光光谱仪耦合，采用可调谐二极管激光吸收光谱（TDLAS）测量叶片光合作用、气孔导度和胞间导度。使用四个气体交换室可将每个叶片样本适应的时间损失最小化。系统操作半自动，叶片温度、湿度和 CO 浓度可通过计算机系统远程调节和监控。与水稻叶片样本的初步结果表明，该系统能够进行高通量测量，这对于获得植物样本生态生理特征更好的代表性是必要的。
