Department of Emergency Surgery, Jining No. 1 People's Hospital, Jining, China.
Eur Rev Med Pharmacol Sci. 2019 Jul;23(14):6045-6052. doi: 10.26355/eurrev_201907_18417.
Recent studies revealed that long non-coding RNAs (lncRNAs) participate in the progression and development of many human diseases. In this work, we are committed to uncovering the association between lncRNA MNX1-AS1 and the development of osteosarcoma.
MNX1-AS1 expression of osteosarcoma cells and tissue samples was detected by Real Time-quantitative Polymerase Chain Reaction (RT-qPCR). Besides, we conducted functional assays including cell proliferation assay, colony formation, and transwell assays. Furthermore, the underlying mechanism including RT-qPCR and Western blot assay was performed.
MNX1-AS1 was higher-expressed in osteosarcoma samples than that in adjacent tissues. The abilities of proliferation and invasion were suppressed after MNX1-AS1 was knocked down in vitro. Moreover, KISS1 expression was upregulated at mRNA and protein level via silence of MNX1-AS1. Furthermore, the underlying mechanism of the development of osteosarcoma were investigated by RT-qPCR and Western blot assay.
Our study demonstrated that MNX1-AS1 could enhance osteosarcoma cell proliferation and invasion by inhibiting KISS1, which might contribute to the therapy for osteosarcoma.
最近的研究表明，长链非编码 RNA（lncRNA）参与了许多人类疾病的进展和发展。在这项工作中，我们致力于揭示 lncRNA MNX1-AS1 与骨肉瘤发展之间的关联。
通过实时定量聚合酶链反应（RT-qPCR）检测骨肉瘤细胞和组织样本中的 MNX1-AS1 表达。此外，我们进行了功能测定，包括细胞增殖测定、集落形成和 Transwell 测定。此外，还进行了包括 RT-qPCR 和 Western blot 测定在内的潜在机制研究。
MNX1-AS1 在骨肉瘤样本中的表达高于相邻组织。在体外敲低 MNX1-AS1 后，增殖和侵袭能力受到抑制。此外，通过沉默 MNX1-AS1，KISS1 的表达在 mRNA 和蛋白水平上均上调。此外，通过 RT-qPCR 和 Western blot 测定研究了骨肉瘤发展的潜在机制。
我们的研究表明，MNX1-AS1 可以通过抑制 KISS1 来增强骨肉瘤细胞的增殖和侵袭，这可能有助于骨肉瘤的治疗。
