• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

长链非编码 RNA RBM5-AS1 促进骨肉瘤细胞的增殖、迁移和侵袭。

LncRNA RBM5-AS1 Promotes Osteosarcoma Cell Proliferation, Migration, and Invasion.

机构信息

The Orthopedic Surgery Department of Guizhou Province Orthopedic Hospital, China.

The Orthopedic Surgery Department of Guiyang Maternal and Child Health-Care Hospital, Guiyang, Guizhou Province 550000, China.

出版信息

Biomed Res Int. 2021 Mar 15;2021:5271291. doi: 10.1155/2021/5271291. eCollection 2021.

DOI:10.1155/2021/5271291
PMID:33816613
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7987434/
Abstract

PURPOSE

Osteosarcoma (Os) is the most frequent malignant tumor of the bone in the pediatric age group, and accumulating evidences show that lncRNAs play a key role in the development of Os. Thus, we investigated the role of RBM5-AS1 and its molecular mechanism.

METHODS

The expression of RBM5-AS1 in Os tissues and cell lines was detected by real-time polymerase chain reaction (QPCR). The effect of RBM5-AS1 on the proliferation of Os cells was detected using CCK8 assays and flow cytometry. The effect of RBM5-AS1 on the migration and invasion of Os cells was detected by transwell assays. And we performed QPCR and western blotting assays to investigate the relationship between RBM5-AS1 and RBM5. Finally, western blotting assays were performed to explore the mechanism of RBM5.

RESULTS

LncRNA RBM5-AS1 was overexpressed in the Os tissues and cell lines. And lncRNA RBM5-AS1 promoted Os cell proliferation, migration, and invasion in vitro and tumor growth in vivo. LncRNA RBM5-AS1 targets RBM5 in Os cells.

CONCLUSION

To sum up, the results showed that lncRNA RBM5-AS1 promotes cell proliferation, migration, and invasion in Os.

摘要

目的

骨肉瘤（OS）是儿童时期最常见的骨恶性肿瘤，越来越多的证据表明长链非编码 RNA（lncRNA）在 OS 的发生发展中起关键作用。因此，我们研究了 RBM5-AS1 的作用及其分子机制。

方法

采用实时聚合酶链反应（QPCR）检测 OS 组织和细胞系中 RBM5-AS1 的表达。用 CCK8 法和流式细胞术检测 RBM5-AS1 对 OS 细胞增殖的影响。用 Transwell 检测 RBM5-AS1 对 OS 细胞迁移和侵袭的影响。进行 QPCR 和 Western blot 检测以研究 RBM5-AS1 与 RBM5 之间的关系。最后，进行 Western blot 检测以探讨 RBM5 的作用机制。

结果

lncRNA RBM5-AS1 在 OS 组织和细胞系中过度表达。lncRNA RBM5-AS1 促进 OS 细胞在体外的增殖、迁移和侵袭以及体内肿瘤的生长。lncRNA RBM5-AS1 在 OS 细胞中靶向 RBM5。

结论

综上所述，这些结果表明 lncRNA RBM5-AS1 促进了 OS 中的细胞增殖、迁移和侵袭。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/899e/7987434/00b58c52fc66/BMRI2021-5271291.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/899e/7987434/518a47920a7e/BMRI2021-5271291.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/899e/7987434/3b74057d206f/BMRI2021-5271291.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/899e/7987434/e4a223935ac3/BMRI2021-5271291.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/899e/7987434/a9855dd50c02/BMRI2021-5271291.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/899e/7987434/02d066fc7b70/BMRI2021-5271291.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/899e/7987434/00b58c52fc66/BMRI2021-5271291.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/899e/7987434/518a47920a7e/BMRI2021-5271291.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/899e/7987434/3b74057d206f/BMRI2021-5271291.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/899e/7987434/e4a223935ac3/BMRI2021-5271291.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/899e/7987434/a9855dd50c02/BMRI2021-5271291.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/899e/7987434/02d066fc7b70/BMRI2021-5271291.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/899e/7987434/00b58c52fc66/BMRI2021-5271291.006.jpg

相似文献

1
LncRNA RBM5-AS1 Promotes Osteosarcoma Cell Proliferation, Migration, and Invasion.长链非编码 RNA RBM5-AS1 促进骨肉瘤细胞的增殖、迁移和侵袭。
Biomed Res Int. 2021 Mar 15;2021:5271291. doi: 10.1155/2021/5271291. eCollection 2021.
2
Silencing of long noncoding RNA SBF2-AS1 inhibits proliferation, migration and invasion and contributes to apoptosis in osteosarcoma cells by upregulating microRNA-30a to suppress FOXA1 expression.长链非编码 RNA SBF2-AS1 的沉默通过上调 microRNA-30a 抑制 FOXA1 表达，抑制骨肉瘤细胞的增殖、迁移和侵袭并促进细胞凋亡。
Cell Cycle. 2019 Oct;18(20):2727-2741. doi: 10.1080/15384101.2019.1656478. Epub 2019 Aug 21.
3
LncRNA DSCAM-AS1 promoted cell proliferation and invasion in osteosarcoma by sponging miR-101.长链非编码 RNA DSCAM-AS1 通过海绵吸附 miR-101 促进骨肉瘤细胞增殖和侵袭。
Eur Rev Med Pharmacol Sci. 2020 Jul;24(14):7709-7717. doi: 10.26355/eurrev_202007_22274.
4
lncRNA GAS8-AS1 downregulates lncRNA UCA1 to inhibit osteosarcoma cell migration and invasion.长链非编码 RNA GAS8-AS1 通过下调 lncRNA UCA1 抑制骨肉瘤细胞迁移和侵袭。
J Orthop Surg Res. 2020 Feb 3;15(1):38. doi: 10.1186/s13018-020-1550-x.
5
LncRNA FOXD2-AS1 knockdown inhibits the resistance of human osteosarcoma cells to cisplatin by inhibiting miR-143 expression.长链非编码 RNA FOXD2-AS1 敲低通过抑制 miR-143 的表达抑制人骨肉瘤细胞对顺铂的耐药性。
Eur Rev Med Pharmacol Sci. 2021 Jan;25(2):678-686. doi: 10.26355/eurrev_202101_24629.
6
LncRNA ADPGK-AS1 regulated cell proliferation, invasion, migration and apoptosis via targeting miR-542-3p in osteosarcoma.长链非编码 RNA ADPGK-AS1 通过靶向 miR-542-3p 调控骨肉瘤细胞的增殖、侵袭、迁移和凋亡。
Eur Rev Med Pharmacol Sci. 2019 Oct;23(20):8751-8760. doi: 10.26355/eurrev_201910_19269.
7
Long noncoding RNA ADIRF antisense RNA 1 upregulates insulin receptor substrate 1 to decrease the aggressiveness of osteosarcoma by sponging microRNA-761.长链非编码 RNA ADIRF 反义 RNA 1 通过海绵吸附 microRNA-761 上调胰岛素受体底物 1 降低骨肉瘤的侵袭性。
Bioengineered. 2022 Feb;13(2):2028-2043. doi: 10.1080/21655979.2021.2019872.
8
FOXP4-AS1 participates in the development and progression of osteosarcoma by downregulating LATS1 via binding to LSD1 and EZH2.FOXP4-AS1 通过与 LSD1 和 EZH2 结合来下调 LATS1，从而参与骨肉瘤的发生和发展。
Biochem Biophys Res Commun. 2018 Aug 25;502(4):493-500. doi: 10.1016/j.bbrc.2018.05.198. Epub 2018 Jun 5.
9
Exerts Oncogenic Role in Osteosarcoma by Promoting Cell Proliferation and Epithelial to Mesenchymal Transition.通过促进细胞增殖和上皮-间质转化在骨肉瘤中发挥致癌作用。
Cancer Biother Radiopharm. 2020 Feb;35(1):58-65. doi: 10.1089/cbr.2019.2885. Epub 2019 Nov 14.
10
Long Noncoding RNA SERTAD2-3 Inhibits Osteosarcoma Proliferation and Migration by Competitively Binding miR-29c.长链非编码RNA SERTAD2-3通过竞争性结合miR-29c抑制骨肉瘤增殖和迁移。
Genet Test Mol Biomarkers. 2020 Feb;24(2):67-72. doi: 10.1089/gtmb.2019.0164.

引用本文的文献

1
Targeting the lncRNA RBM5-AS1/GCN5 axis under fasting conditions reprograms Glycolysis and induces apoptosis in ovarian cancer cells.在禁食条件下靶向lncRNA RBM5-AS1/GCN5轴可重编程糖酵解并诱导卵巢癌细胞凋亡。
Mol Biol Rep. 2025 Jul 9;52(1):691. doi: 10.1007/s11033-025-10800-z.
2
Roles of lncRNAs in childhood cancer: Current landscape and future perspectives.长链非编码RNA在儿童癌症中的作用：现状与未来展望
Front Oncol. 2023 Feb 27;13:1060107. doi: 10.3389/fonc.2023.1060107. eCollection 2023.
3
Bioinformatics analysis of lncRNAs in the occurrence and development of osteosarcoma.

本文引用的文献

1
Long non-coding RNA RBM5-AS1 promotes the aggressive behaviors of oral squamous cell carcinoma by regulation of miR-1285-3p/YAP1 axis.长链非编码 RNA RBM5-AS1 通过调控 miR-1285-3p/YAP1 轴促进口腔鳞状细胞癌的侵袭行为。
Biomed Pharmacother. 2020 Mar;123:109723. doi: 10.1016/j.biopha.2019.109723. Epub 2019 Dec 20.
2
Identification of Prognostic and Predictive Osteosarcoma Biomarkers.骨肉瘤预后和预测生物标志物的鉴定
Med Sci (Basel). 2019 Feb 11;7(2):28. doi: 10.3390/medsci7020028.
3
Current and Emerging Targets in Immunotherapy for Osteosarcoma.
骨肉瘤发生发展过程中lncRNAs的生物信息学分析
Transl Pediatr. 2022 Jul;11(7):1182-1198. doi: 10.21037/tp-22-253.
4
dbEssLnc: A manually curated database of human and mouse essential lncRNA genes.dbEssLnc：一个人工整理的人类和小鼠必需长链非编码RNA基因数据库。
Comput Struct Biotechnol J. 2022 May 23;20:2657-2663. doi: 10.1016/j.csbj.2022.05.043. eCollection 2022.
5
Hypoxia-induced lncRNA RBM5-AS1 promotes tumorigenesis via activating Wnt/β-catenin signaling in breast cancer.低氧诱导的长链非编码 RNA RBM5-AS1 通过激活乳腺癌中的 Wnt/β-连环蛋白信号通路促进肿瘤发生。
Cell Death Dis. 2022 Feb 2;13(2):95. doi: 10.1038/s41419-022-04536-y.
骨肉瘤免疫治疗的当前及新出现的靶点
J Oncol. 2019 Jan 1;2019:7035045. doi: 10.1155/2019/7035045. eCollection 2019.
4
Role of RNA-binding protein 5 in the diagnosis and chemotherapeutic response of lung cancer.RNA结合蛋白5在肺癌诊断及化疗反应中的作用
Oncol Lett. 2019 Feb;17(2):2013-2019. doi: 10.3892/ol.2018.9818. Epub 2018 Dec 10.
5
RBM5-AS1 participates in fracture healing and inhibits apoptosis of bone cells through the up-regulation of β-catenin.RBM5-AS1 通过上调β-连环蛋白参与骨折愈合并抑制骨细胞凋亡。
Eur Rev Med Pharmacol Sci. 2018 Aug;22(16):5091-5097. doi: 10.26355/eurrev_201808_15702.
6
MiR-1297 suppresses pancreatic cancer cell proliferation and metastasis by targeting MTDH.miR-1297 通过靶向 MTDH 抑制胰腺癌细胞增殖和转移。
Mol Cell Probes. 2018 Aug;40:19-26. doi: 10.1016/j.mcp.2018.06.003. Epub 2018 Jun 13.
7
LncRNA SNHG12 promotes tumorigenesis and metastasis in osteosarcoma by upregulating Notch2 by sponging miR-195-5p.长链非编码RNA SNHG12通过吸附miR-195-5p上调Notch2来促进骨肉瘤的肿瘤发生和转移。
Biochem Biophys Res Commun. 2018 Jan 8;495(2):1822-1832. doi: 10.1016/j.bbrc.2017.12.047. Epub 2017 Dec 9.
8
Current and future therapeutic approaches for osteosarcoma.骨肉瘤的当前及未来治疗方法
Expert Rev Anticancer Ther. 2018 Jan;18(1):39-50. doi: 10.1080/14737140.2018.1413939. Epub 2017 Dec 14.
9
Regulating RNA Binding Motif 5 Gene Expression- A Novel Therapeutic Target for Lung Cancer.调控RNA结合基序5基因表达——肺癌的新型治疗靶点
J Environ Pathol Toxicol Oncol. 2017;36(2):99-105. doi: 10.1615/JEnvironPatholToxicolOncol.2017019366.
10
Application of Long Noncoding RNAs in Osteosarcoma: Biomarkers and Therapeutic Targets.长链非编码RNA在骨肉瘤中的应用：生物标志物与治疗靶点
Cell Physiol Biochem. 2017;42(4):1407-1419. doi: 10.1159/000479205. Epub 2017 Jul 17.