长链非编码 RNA RBM5-AS1 促进骨肉瘤细胞的增殖、迁移和侵袭。
LncRNA RBM5-AS1 Promotes Osteosarcoma Cell Proliferation, Migration, and Invasion.
机构信息
The Orthopedic Surgery Department of Guizhou Province Orthopedic Hospital, China.
The Orthopedic Surgery Department of Guiyang Maternal and Child Health-Care Hospital, Guiyang, Guizhou Province 550000, China.
出版信息
Biomed Res Int. 2021 Mar 15;2021:5271291. doi: 10.1155/2021/5271291. eCollection 2021.
PURPOSE
Osteosarcoma (Os) is the most frequent malignant tumor of the bone in the pediatric age group, and accumulating evidences show that lncRNAs play a key role in the development of Os. Thus, we investigated the role of RBM5-AS1 and its molecular mechanism.
METHODS
The expression of RBM5-AS1 in Os tissues and cell lines was detected by real-time polymerase chain reaction (QPCR). The effect of RBM5-AS1 on the proliferation of Os cells was detected using CCK8 assays and flow cytometry. The effect of RBM5-AS1 on the migration and invasion of Os cells was detected by transwell assays. And we performed QPCR and western blotting assays to investigate the relationship between RBM5-AS1 and RBM5. Finally, western blotting assays were performed to explore the mechanism of RBM5.
RESULTS
LncRNA RBM5-AS1 was overexpressed in the Os tissues and cell lines. And lncRNA RBM5-AS1 promoted Os cell proliferation, migration, and invasion in vitro and tumor growth in vivo. LncRNA RBM5-AS1 targets RBM5 in Os cells.
CONCLUSION
To sum up, the results showed that lncRNA RBM5-AS1 promotes cell proliferation, migration, and invasion in Os.
目的
骨肉瘤（OS）是儿童时期最常见的骨恶性肿瘤，越来越多的证据表明长链非编码 RNA（lncRNA）在 OS 的发生发展中起关键作用。因此，我们研究了 RBM5-AS1 的作用及其分子机制。
方法
采用实时聚合酶链反应（QPCR）检测 OS 组织和细胞系中 RBM5-AS1 的表达。用 CCK8 法和流式细胞术检测 RBM5-AS1 对 OS 细胞增殖的影响。用 Transwell 检测 RBM5-AS1 对 OS 细胞迁移和侵袭的影响。进行 QPCR 和 Western blot 检测以研究 RBM5-AS1 与 RBM5 之间的关系。最后，进行 Western blot 检测以探讨 RBM5 的作用机制。
结果
lncRNA RBM5-AS1 在 OS 组织和细胞系中过度表达。lncRNA RBM5-AS1 促进 OS 细胞在体外的增殖、迁移和侵袭以及体内肿瘤的生长。lncRNA RBM5-AS1 在 OS 细胞中靶向 RBM5。
结论
综上所述，这些结果表明 lncRNA RBM5-AS1 促进了 OS 中的细胞增殖、迁移和侵袭。
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