Department of Otolaryngology-Head and Neck Surgery, Xiaogan Hospital Affiliated to Wuhan University of Science and Technology, Xiaogan, China.
Eur Rev Med Pharmacol Sci. 2019 Jul;23(14):6234-6241. doi: 10.26355/eurrev_201907_18442.
Long noncoding RNAs (lncRNAs) have been reported to participate in the progression and development of many human diseases. In this study, we are committed to uncover the potential function of lncRNA Nuclear Enriched Abundant Transcript 1 (NEAT1) in the development of laryngocarcinoma.
LncRNA NEAT1 expression in laryngocarcinoma cells and 54 paired laryngocarcinoma samples was detected by Real-time quantitative polymerase chain reaction (RT-qPCR). Furthermore, the regulatory effects of NEAT1 on the proliferation and metastasis of laryngocarcinoma cells were evaluated. Biological role of NEAT1/miR-29a-3p axis was finally explored in regulating the progression of laryngocarcinoma.
NEAT1 was upregulated in laryngocarcinoma tissues and cell lines. NEAT1 knockdown suppressed growth and invasive abilities in laryngocarcinoma cells, while overexpression of NEAT1 enhanced such abilities. Further experiments showed that miR-29a-3p was directly targeted by NEAT1, and participated in NEAT-mediated progression of laryngocarcinoma.
NEAT1 is a novel oncogene in laryngocarcinoma and could enhance growth and invasion of laryngocarcinoma cells by targeting miR-29a-3p.
长链非编码 RNA（lncRNAs）已被报道参与许多人类疾病的进展和发展。在这项研究中，我们致力于揭示 lncRNA 核丰富丰富转录物 1（NEAT1）在喉癌发展中的潜在功能。
通过实时定量聚合酶链反应（RT-qPCR）检测喉癌细胞和 54 对喉癌样本中的 lncRNA NEAT1 表达。此外，评估了 NEAT1 对喉癌细胞增殖和转移的调节作用。最后，探索了 NEAT1/miR-29a-3p 轴在调节喉癌进展中的生物学作用。
NEAT1 在喉癌组织和细胞系中上调。NEAT1 敲低抑制了喉癌细胞的生长和侵袭能力，而 NEAT1 的过表达增强了这种能力。进一步的实验表明，miR-29a-3p 是由 NEAT1 直接靶向的，并参与了 NEAT 介导的喉癌进展。
NEAT1 是喉癌中的一种新型癌基因，可通过靶向 miR-29a-3p 增强喉癌细胞的生长和侵袭。
