Department of General Surgery, Beijing Bo'ai Hospital, China Rehabilitation Research Center, Beijing, China.
Eur Rev Med Pharmacol Sci. 2019 Nov;23(22):9857-9862. doi: 10.26355/eurrev_201911_19549.
Recent studies have proved that long noncoding RNAs (lncRNAs) act as an important role in many diseases. In this research, lncRNA XIST was explored to identify how it functions in the development of hepatocellular carcinoma (HCC).
Real Time-quantitative Polymerase Chain Reaction (RT-qPCR) was utilized to detect XIST expression in HCC patients. Then, we conducted Cell Counting Kit-8 (CCK-8) assay and colony formation assays in vitro. Furthermore, mechanism assays and the interaction between XIST and miR-200b-3p were conducted.
By comparing with XIST expression in adjacent tissues, the XIST expression level was significantly higher in HCC samples. Moreover, functional assays showed that the cell growth ability of HCC cells was inhibited after XIST was silenced in vitro, and tumor formation was inhibited after XIST was silenced in vivo. Further experiments showed that miR-200b-3p was directly targeted by XIST.
Above results suggest that XIST could enhance the cell growth ability of HCC by targeting miR-200b-3p, which suggest that XIST may be a potential therapeutic target in HCC.
最近的研究证明长非编码 RNA（lncRNAs）在许多疾病中起着重要作用。在这项研究中，探索了 lncRNA XIST，以确定它在肝细胞癌（HCC）发展中的作用机制。
实时定量聚合酶链反应（RT-qPCR）用于检测 HCC 患者中 XIST 的表达。然后，我们进行了细胞计数试剂盒-8（CCK-8）测定和体外集落形成测定。此外，还进行了机制测定和 XIST 与 miR-200b-3p 之间的相互作用。
与相邻组织中的 XIST 表达相比，HCC 样本中的 XIST 表达水平明显升高。此外，功能测定表明，体外沉默 XIST 后 HCC 细胞的细胞生长能力受到抑制，体内沉默 XIST 后肿瘤形成受到抑制。进一步的实验表明，XIST 可以直接靶向 miR-200b-3p。
上述结果表明，XIST 通过靶向 miR-200b-3p 增强 HCC 的细胞生长能力，这表明 XIST 可能是 HCC 的潜在治疗靶点。
