Department of Pharmacy, The 940th Hospital of Joint Logistic Support Force, PLA, Lanzhou, China.
Key Laboratory of the Prevention and Treatment for Injure in Plateau of PLA, Lanzhou, China.
Phytother Res. 2019 Oct;33(10):2692-2701. doi: 10.1002/ptr.6443. Epub 2019 Jul 30.
Verbascoside is a water-soluble natural phenylethanoid glycoside and distributes widely in plants. It has been proved with antioxidant, neuroprotective, anti-inflammatory, antibacterial, and immunomodulatory bioactivities. In this experiment, the effect and mechanism of verbascoside on hypoxic memory injury were studied in a low-pressure and low-oxygen chamber. Verbascoside (50, 150, and 300 mg/kg) was intragastrically administered once a day for 7 days. On the fourth day, rats were placed in the chamber to simulate a 7,500 m high-altitude environment The eight-arm maze was used to test the memory ability. The levels of MDA, GSH, and T-SOD in plasma, brain-NH, and hippocampus were detected. The mRNA expression of mTOR, P70S6K, and 4E-BP1 in the hippocampus tissue was determined by PCR. The protein expression of P-mTOR, P-P70S6K, P-4E-BP1, and Cleaved Caspase-3 in the hippocampus tissue was determined by western blot. The results indicated that administration with verbascoside could obviously reduce the working memory error, reference memory error, total errors, and total time; relieve the neuron damage in CA region of the hippocampus; and decrease the oxidative stress correlation enzyme activity in plasma, brain, and hippocampus. The amelioration of verbascoside on high altitude-induced memory impairment may be associated with the adjustment of oxidative stress and mTOR signaling pathway.
毛蕊花糖苷是一种水溶性苯乙醇苷类天然产物，广泛分布于植物中。它具有抗氧化、神经保护、抗炎、抗菌和免疫调节等生物活性。本实验在低压低氧舱中研究了毛蕊花糖苷对低氧记忆损伤的作用及机制。毛蕊花糖苷（50、150 和 300mg/kg）每天灌胃 1 次，连续 7 天。第 4 天，将大鼠置于舱内模拟 7500m 高空环境，采用八臂迷宫测试大鼠记忆能力。检测血浆、脑-NH3 和海马中 MDA、GSH 和 T-SOD 的水平。通过 PCR 测定海马组织中 mTOR、P70S6K 和 4E-BP1 的 mRNA 表达。通过 Western blot 测定海马组织中 P-mTOR、P-P70S6K、P-4E-BP1 和 Cleaved Caspase-3 的蛋白表达。结果表明，毛蕊花糖苷给药可明显减少大鼠的工作记忆错误、参考记忆错误、总错误和总时间；减轻海马 CA 区神经元损伤；降低血浆、脑和海马中氧化应激相关酶的活性。毛蕊花糖苷改善高原引起的记忆损伤可能与调节氧化应激和 mTOR 信号通路有关。
