Nagoya University, Department of Electronics, Nagoya, Aichi, Japan.
J Biomed Opt. 2019 Jul;24(7):1-4. doi: 10.1117/1.JBO.24.7.070502.
We present three-dimensional (3-D) high-resolution spectral-domain optical coherence microscopy (SD-OCM) by using a supercontinuum (SC) fiber laser source with 300-nm spectral bandwidth (full-width at half-maximum) in the 1700-nm spectral band. By using low-coherence interferometry with SC light and a confocal detection scheme, we realized lateral and axial resolutions of 3.4 and 3.8 μm in tissue (n = 1.38), respectively. This is, to the best of our knowledge, the highest 3-D spatial resolution reported among those of Fourier-domain optical coherence imaging techniques in the 1700-nm spectral band. In our SD-OCM, to enhance the imaging depth, a full-range method was implemented, which suppressed the formation of a coherent ghost image and allowed us to set the zero-delay position inside the samples. We demonstrated the 3-D high-resolution imaging capability of 1700-nm SD-OCM through the measurement of an interference signal from a mirror surface and imaging of a single 200-nm polystyrene bead and a pig thyroid gland. Deep tissue imaging at a depth of up to 1.8 mm was also demonstrated. This is the first demonstration of 3-D high-resolution SD-OCM in the 1700-nm spectral band.
我们提出了一种基于超连续（SC）光纤激光源的三维（3-D）高分辨率光谱域光相干显微镜（SD-OCM），该激光源在 1700nm 光谱带内具有 300nm 光谱带宽（半峰全宽）。通过使用 SC 光的低相干干涉测量和共焦检测方案，我们在组织中实现了 3.4μm 的横向分辨率和 3.8μm 的轴向分辨率（n=1.38）。据我们所知，这是在 1700nm 光谱带中，傅里叶域光相干成像技术中报道的最高 3-D 空间分辨率。在我们的 SD-OCM 中，为了增强成像深度，我们采用了全范围方法，该方法抑制了相干鬼像的形成，并允许我们在样品内部设置零延迟位置。我们通过测量镜面的干涉信号以及对单个 200nm 聚苯乙烯珠和猪甲状腺的成像，展示了 1700nm SD-OCM 的 3-D 高分辨率成像能力。还展示了深度达 1.8mm 的深层组织成像。这是在 1700nm 光谱带中首次演示 3-D 高分辨率 SD-OCM。
