Biosensor Technologies , AIT-Austrian Institute of Technology GmbH , Konrad-Lorenz-Straße 24 , 3430 Tulln an der Donau , Austria.
DWI - Leibniz Institute for Interactive Materials and Institute of Technical and Macromolecular Chemistry , RWTH Aachen University , Forckenbeckstraße 50 , 52074 Aachen , Germany.
ACS Sens. 2019 Aug 23;4(8):2109-2116. doi: 10.1021/acssensors.9b00827. Epub 2019 Aug 14.
A compact optical biosensor for direct detection of thrombin in human blood plasma (HBP) is reported. This biosensor platform is based on wavelength spectroscopy of diffraction-coupled surface plasmons on a chip with a periodically corrugated gold film that carries an antifouling thin polymer layer consisting of poly[(-(2-hydroxypropyl)methacrylamide)--(carboxybetaine methacrylamide)] (poly(HPMA--CBMAA)) brushes. This surface architecture provides superior resistance to nonspecific and irreversible adsorption of abundant compounds in the analyzed HBP samples in comparison to standard surface modifications. The carboxylate groups along the polymer brushes were exploited for the covalent immobilization of aptamer ligands. These ligands were selected to specifically capture the target thrombin analyte from the analyzed HBP sample in a way that does not activate the coagulatory process at the biosensor surface with poly(HPMA--CBMAA) brushes. Direct label-free analysis of thrombin in the medically relevant concentration range (1-20 nM) is demonstrated without the need for diluting the HBP samples or using additional steps for signal enhancement. The reported platform constitutes the first step toward a portable and sensitive point-of-care device for direct detection of thrombin in human blood.
一种用于直接检测人血浆（HBP）中凝血酶的紧凑型光学生物传感器被报道。该生物传感器平台基于芯片上的衍射耦合表面等离子体的波长光谱，该芯片带有周期性波纹的金膜，该金膜带有抗污染的薄聚合物层，由聚[（-（2-羟丙基）甲基丙烯酰胺）-（羧基甜菜碱甲基丙烯酰胺）]（聚（HPMA--CBMAA）刷组成。与标准表面修饰相比，这种表面结构提供了对分析 HBP 样品中丰富化合物的非特异性和不可逆吸附的优异抵抗力。聚合物刷上的羧酸基团被用于共价固定适体配体。这些配体被选择用于以不激活生物传感器表面上的凝血过程的方式从分析的 HBP 样品中特异性捕获靶标凝血酶分析物，其中聚合物刷的 poly(HPMA--CBMAA)。在不需要稀释 HBP 样品或使用额外的信号增强步骤的情况下，在医学相关浓度范围内（1-20 nM）直接对凝血酶进行无标记分析。该报告的平台是朝着用于直接检测人血中凝血酶的便携式和灵敏的即时检测设备迈出的第一步。
