Institute of Jeju Microbial Resources, BioPS Co., Ltd., Jeju 63243, Republic of Korea.
Department of Biological Sciences and Biotechnology, Hannam University, Daejon 34054, Republic of Korea.
Int J Syst Evol Microbiol. 2021 Jul;71(7). doi: 10.1099/ijsem.0.004890.
Two novel actinobacterial strains, designated C9-5 and C3-43, were isolated from soil samples of a cave in Jeju Island, Republic of Korea, and subjected to taxonomic study by a polyphasic approach. The organisms exhibited a typical rod-coccus developmental cycle during growth and grew at 10-30 °C, pH 5-9 and 0-3 % (w/v) NaCl. In 92 single-copy core gene sequence analysis, strain C9-5 was loosely associated with , albeit sharing low 16S rRNA gene sequence similarity (97.4 %). A combination of morphological and chemotaxonomic characteristics supported assignment with the genus . With respect to 16S rRNA gene sequence similarity, the novel isolates showed the highest identity to the type strain of (98.7 % sequence similarity), followed by (98.5 %) and (98.4 %).The average nucleotide identity and digital DNA-DNA hybridization values between strain C9-5 and members of the genus were ≤81.5 and ≤37.1 %, respectively. A set of physiological and chemotaxonomic properties together with overall genomic relatedness differentiated the novel isolates from members of the genus , for which the name sp. nov. is proposed. The type strain is C9-5 (=KACC 19822=DSM 107558). Based on genome analysis performed here, it is also proposed that Su . 2015 is a later heterotypic synonym of Yoon . 2000, Xu . 2007 and Li . 2004 are later heterotypic synonyms of (Gray and Thornton 1928) Goodfellow and Alderson 1979 (Approved Lists 1980), and Briglia . 1996 and Ghosh . 2006 are later heterotypic synonyms of Klatte . 1995.
两株新的放线菌菌株,命名为 C9-5 和 C3-43,从韩国济州岛洞穴的土壤样本中分离出来,并通过多相分类法进行了分类学研究。这些生物体在生长过程中表现出典型的杆状-球菌发育周期,可在 10-30°C、pH 值 5-9 和 0-3%(w/v)NaCl 下生长。在 92 个单拷贝核心基因序列分析中,菌株 C9-5 与 松散相关,尽管 16S rRNA 基因序列相似性较低(97.4%)。形态学和化学分类特征的结合支持将其归类于属 。就 16S rRNA 基因序列相似性而言,新型分离株与 (98.7%序列相似性)的模式株最为相似,其次是 (98.5%)和 (98.4%)。与属内其他成员相比,菌株 C9-5 的平均核苷酸同一性和数字 DNA-DNA 杂交值分别为≤81.5%和≤37.1%。一组生理和化学分类特性以及整体基因组相关性将新型分离株与属内成员区分开来,因此提议将其命名为 sp. nov.。模式株为 C9-5(=KACC 19822=DSM 107558)。基于此处进行的基因组分析,还提议将 Su. 2015 作为 Yoon. 2000、Xu. 2007 和 Li. 2004 的后同型异名,以及 Briglia. 1996 和 Ghosh. 2006 作为 Klatte. 1995 的后同型异名。