Department of Bio-Organic Chemistry, Institute for Molecules and Materials , Radboud University , 6525 AJ Nijmegen , the Netherlands.
Department of Regenerative Biomaterials , Radboudumc , 6525 EX Nijmegen , the Netherlands.
Biomacromolecules. 2019 Aug 12;20(8):2913-2921. doi: 10.1021/acs.biomac.9b00104. Epub 2019 Jul 31.
A library of poly(2-oxazoline)s functionalized with controllable amounts of alendronate, hydroxyl, and carboxylic acid side groups was successfully synthesized to create novel polymers with tunable affinity for calcium cations. The affinity of alendronate-containing polymers for calcium cations was quantified using isothermal titration calorimetry. Thermodynamic measurements revealed that the Ca-binding affinity of these polymers increased linearly with the amount of alendronate functionalization, up to values ( = 2.4 × 10 M) that were about 120-fold higher than those for previously reported polymers. The calcium-binding capacity of alendronate-functionalized poly(2-oxazoline)s was exploited to form robust hydrogel networks cross-linked using reversible physical bonds. Oscillatory rheology showed that these hydrogels recovered more than 100% of their initial storage modulus after severe network destruction. The versatile synthesis of alendronate-functionalized polymers and their strong and tunable affinity for calcium cations render these polymers promising candidates for various biomedical applications.
成功合成了一种带有可控制数量的阿仑膦酸盐、羟基和羧酸侧基的聚（2-恶唑啉）库，以创造对钙离子具有可调亲和力的新型聚合物。使用等温滴定量热法定量测定含阿仑膦酸盐聚合物对钙离子的亲和力。热力学测量表明，这些聚合物对钙离子的结合亲和力随阿仑膦酸盐官能化程度呈线性增加，达到的值（=2.4×10 M）比以前报道的聚合物高约 120 倍。阿仑膦酸盐功能化聚（2-恶唑啉）的钙离子结合能力被利用来形成使用可逆物理键交联的坚固水凝胶网络。振荡流变学表明，这些水凝胶在严重的网络破坏后恢复了超过其初始储能模量的 100%。阿仑膦酸盐功能化聚合物的多功能合成及其对钙离子的强而可调的亲和力使这些聚合物成为各种生物医学应用的有前途的候选物。
