Sevik Mehmet A
Department of Plant Protection, Faculty of Agriculture, University of Ondokuz Mayis, 55139, Atakum, Samsun, Turkey.
An Acad Bras Cienc. 2019 Jul 29;91(3):e20180224. doi: 10.1590/0001-3765201920180224.
Virus diseases of cool season vegetable crops (mainly cabbage, white and red head cabbage, broccoli, kale, radish, rocket salad, garden cress, and turnip) were surveyed in Bafra Plain, Turkey during winter 2017, and 2018. Leaf samples were collected from different species of the Brassicaceae family showing mosaic, mottling, necrotic spots, malformation, and chlorosis symptoms. These samples were tested for the presence of Cauliflower mosaic virus (CaMV), Cucumber mosaic virus (CMV), Beet western yellows virus (BWYV), Radish mosaic virus (RaMV), Turnip mosaic virus (TuMV), Turnip yellow mosaic virus (TYMV), and Turnip yellows virus (TuYV) by biological and serological methods. A total of 455 samples were collected from cole crop fields and tested for the seven viruses by double-antibody sandwich ELISA using specific polyclonal antibodies. According to the results, out of these, 7 % of the samples were infected by at least one of these viruses. TuMV was the most prevalent virus detected in cole crops. TuMV, CaMV, and CMV were detected in 3 %, 2 %, and 2 % of infected samples, respectively, and the infection rate of these three viruses changed significantly among Brassica species.
2017年冬季和2018年期间，在土耳其的巴夫拉平原对冷季蔬菜作物（主要是卷心菜、白球甘蓝和红球甘蓝、西兰花、羽衣甘蓝、萝卜、芝麻菜、独行菜和芜菁）的病毒病进行了调查。从十字花科不同物种中采集了表现出花叶、斑驳、坏死斑点、畸形和黄化症状的叶片样本。通过生物学和血清学方法对这些样本进行检测，以确定是否存在花椰菜花叶病毒（CaMV）、黄瓜花叶病毒（CMV）、甜菜西方黄化病毒（BWYV）、萝卜花叶病毒（RaMV）、芜菁花叶病毒（TuMV）、芜菁黄花叶病毒（TYMV）和芜菁黄化病毒（TuYV）。从油菜作物田共采集了455个样本，并使用特异性多克隆抗体通过双抗体夹心ELISA对这七种病毒进行检测。根据结果，其中7%的样本至少感染了这些病毒中的一种。TuMV是在油菜作物中检测到的最普遍的病毒。TuMV、CaMV和CMV分别在3%、2%和2%的感染样本中被检测到，这三种病毒的感染率在芸苔属物种之间有显著变化。
