Chabannes D, Le Mauff B, Hallet M M, Yacques Y, Soulillou J P
Institut National pour la Recherche Scientifique et Médicale, Unite 211, Faculté de Médecine, Nantes, France.
Transplantation. 1988 Aug;46(2 Suppl):97S-100S. doi: 10.1097/00007890-198808001-00018.
The effect of CsA on antigen-induced IL-2 receptor expression was studied on a human T lymphocyte clone (4AS) obtained from cells infiltrating a rejected human kidney. Stimulation of 4AS clone cells with specific antigen (D.BLCL) was strongly inhibited by CsA (50% inhibition of tritiated thymidine uptake at about 12.5 ng/ml). Addition of recombinant IL-2 only partially restored 4AS growth inhibition, suggesting that another antigen-induced activation signal such as IL-2-receptor expression could be impaired by CsA. Using 125I-labeled human recombinant IL-2 and 125I-labeled 33B3.1 (a MoAb directed against TAC antigen), we found that expression of both high and low affinity sites was decreased when clone cells were stimulated with D.BLCL in the presence of CsA and exogenous IL-2 (about 50% inhibition in the presence of 500 ng/ml of CsA). Northern blot analysis of IL-2-receptor m.RNA (TAC antigen m.RNA) showed that inhibition occurred at least in part at the pretranscriptional level.
研究了环孢素A（CsA）对从被排斥的人肾浸润细胞中获得的人T淋巴细胞克隆（4AS）上抗原诱导的白细胞介素-2（IL-2）受体表达的影响。用特异性抗原（D.BLCL）刺激4AS克隆细胞时，CsA强烈抑制该刺激（在约12.5 ng/ml时对氚标记胸腺嘧啶核苷摄取的抑制率为50%）。添加重组IL-2仅部分恢复4AS的生长抑制，这表明诸如IL-2受体表达等另一种抗原诱导的激活信号可能被CsA损害。使用125I标记的人重组IL-2和125I标记的33B3.1（一种针对TAC抗原的单克隆抗体），我们发现当克隆细胞在CsA和外源性IL-2存在下用D.BLCL刺激时，高亲和力和低亲和力位点的表达均降低（在500 ng/ml CsA存在下约50%的抑制率）。对IL-2受体mRNA（TAC抗原mRNA）的Northern印迹分析表明，抑制至少部分发生在转录前水平。
