cobas 4800 系统中人免疫缺陷病毒 1 型（HIV-1）检测的比较：cobas 4800 系统中的 cobas 人免疫缺陷病毒 1 型（HIV-1）检测与 COBAS AmpliPrep/COBAS TaqMan HIV-1 检测和 Abbott RealTime HIV-1 检测的比较，以及 cobas HIV-1 的性能评估。
Comparison of the cobas Human Immunodeficiency Virus 1 (HIV-1) Test Using the cobas 4800 System With COBAS AmpliPrep/COBAS TaqMan HIV-1 Test and Abbott RealTime HIV-1 Assay and Performance Evaluation of cobas HIV-1.
机构信息
Department of Laboratory Medicine, Busan, South Korea.
Biomedical Research Institute, Pusan National University Hospital, Busan, South Korea.
出版信息
Am J Clin Pathol. 2019 Oct 7;152(5):558-562. doi: 10.1093/ajcp/aqz075.
OBJECTIVES
To compare quantified human immunodeficiency virus type 1 (HIV-1) viral load quantified using the cobas HIV-1 test with that obtained using the CAP/CTM HIV-1 and Abbott RealTime HIV-1 tests and evaluate the performance of cobas HIV-1 using the cobas 4800 system.
METHODS
Clinical samples (n = 123) were quantitatively analyzed using the CAP/CTM HIV-1, Abbott RealTime HIV-1, and cobas HIV-1 tests, and the precision, linearity, and limit of detection of the cobas HIV-1 test were evaluated.
RESULTS
Comparable results were obtained by both methods: ([log CAP/CTM HIV-1 value] = 0.979 * [log cobas HIV-1 test value] + 0.034) and ([log Abbott RealTime HIV-1 value] = 0.985 * [log cobas HIV-1 test value] + 0.027). cobas HIV-1 test results for 89.4% and 93.5% were within 0.5 log10 IU/mL of the CAP/CTM HIV-1 and Abbott RealTime HIV-1 results, respectively.
CONCLUSIONS
The cobas HIV-1 test showed good performance, and its results correlated well with those of other two tests.
目的
比较使用 cobas HIV-1 检测与 CAP/CTM HIV-1 和 Abbott RealTime HIV-1 检测定量检测的人类免疫缺陷病毒 1 型（HIV-1）病毒载量，并评估 cobas HIV-1 检测在 cobas 4800 系统上的性能。
方法
使用 CAP/CTM HIV-1、 Abbott RealTime HIV-1 和 cobas HIV-1 检测对临床样本（n=123）进行定量分析，评估 cobas HIV-1 检测的精密度、线性和检测限。
结果
两种方法均得到可比的结果：([log CAP/CTM HIV-1 值] = 0.979 * [log cobas HIV-1 检测值] + 0.034) 和 ([log Abbott RealTime HIV-1 值] = 0.985 * [log cobas HIV-1 检测值] + 0.027)。cobas HIV-1 检测结果与 CAP/CTM HIV-1 和 Abbott RealTime HIV-1 结果的 89.4%和 93.5%分别在 0.5 log10 IU/mL 以内。
结论
cobas HIV-1 检测表现出良好的性能，其结果与其他两种检测方法相关性良好。
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