Beier Ross C, Byrd J Allen, Caldwell Denise, Andrews Kathleen, Crippen Tawni L, Anderson Robin C, Nisbet David J
United States Department of Agriculture, Agricultural Research Service, Southern Plains Agricultural Research Center, Food and Feed Safety Research Unit, College Station, TX 77845-4988, USA.
Microorganisms. 2019 Jul 30;7(8):223. doi: 10.3390/microorganisms7080223.
is a bacterium that causes major diarrheal disease worldwide and is also one of the top five foodborne pathogens encountered in the United States. Poultry is a major source of , and a high-risk factor for contracting campylobacteriosis. Organic acids are used in the United States during food animal processing for removal of bacterial contamination from animal carcasses. Six organic acids were evaluated in inhibition studies of 96 strains obtained from shoe covers used in broiler chicken houses at different poultry farms in several states by determining the susceptibilities of the strains, along with the pH values at the molar minimum inhibitory concentrations (MICs). The undissociated and dissociated organic acid concentrations were calculated at the MICs with the Henderson-Hasselbalch equation. The results for the 96 strains were treated similarly for each different organic acid. inhibition did correlate with the dissociated organic acids, but did not correlate with pH or with the undissociated organic acids. When the concentrations of dissociated organic acids decreased, the strains were not disinfected. A carcass wash using organic acids should have the concentration of dissociated acid species carefully controlled. It is suggested to maintain a dissociated acid concentration for propionic, l-lactic, formic, citric, butyric, and acetic acids at 24, 40, 36, 21, 23, and 25 mM, respectively, and at these dissociated organic acid levels an acid wash would be expected to remove or inhibit 97% or more of the bacteria studied here. However, studies must be undertaken to confirm that the suggested concentrations of dissociated organic acids are adequate to remove bacteria in the field vs. the laboratory. Due to propionate, l-lactate, formate, butyrate, and acetate being utilized by , these organic acids may not be appropriate for use as a carcass wash to remove surface contamination. Of all tested organic acids, dissociated citric acid was the most efficient at inhibiting .
是一种在全球范围内引发严重腹泻疾病的细菌，也是美国遇到的五大食源性病原体之一。家禽是 的主要来源，也是感染弯曲杆菌病的高危因素。在美国，有机酸用于食用动物加工过程中，以去除动物 carcasses 上的细菌污染。通过测定从几个州不同家禽养殖场的肉鸡舍使用的鞋套中获得的96株 菌株的敏感性，以及在摩尔最低抑菌浓度（MIC）下的pH值，对六种有机酸进行了抑制研究。使用亨德森 - 哈塞尔巴尔赫方程计算MIC下未解离和解离的有机酸浓度。对于每种不同的有机酸，对96株 菌株的结果进行类似处理。 抑制作用确实与解离的有机酸相关，但与pH值或未解离的有机酸无关。当解离的有机酸浓度降低时， 菌株未被消毒。使用有机酸进行 carcass 清洗时，应仔细控制解离酸种类的浓度。建议分别将丙酸、L - 乳酸、甲酸、柠檬酸、丁酸和乙酸的解离酸浓度维持在24、40、36、21、23和25 mM，在这些解离有机酸水平下，酸洗有望去除或抑制此处研究的97%或更多的 细菌。然而，必须进行研究以确认建议的解离有机酸浓度是否足以在实际环境中与实验室中去除 细菌。由于丙酸、L - 乳酸、甲酸、丁酸和乙酸会被 利用，这些有机酸可能不适用于作为 carcass 清洗以去除 表面污染。在所有测试的有机酸中，解离的柠檬酸在抑制 方面最有效。
