基于通用筛查 PCR 从 L 片段序列预测普马拉病毒感染的空间来源。

Prediction of the Spatial Origin of Puumala Virus Infections Using L Segment Sequences Derived from a Generic Screening PCR.

机构信息

Institute of Virology, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 10117 Berlin, Germany.

Institute of Virology, Biomedical Research Center, Slovak Academy of Sciences, 84505 Bratislava, Slovakia.

出版信息

Viruses. 2019 Jul 30;11(8):694. doi: 10.3390/v11080694.

DOI:10.3390/v11080694

PMID:31366168

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6724045/

Abstract

To screen diagnostic specimens for the presence of hantavirus genomes or to identify new hantaviruses in nature, the pan-hanta L-PCR assay, a broadly reactive nested reverse transcription polymerase chain reaction (RT-PCR) assay targeting the L segment, is highly preferred over other assays because of its universality and high sensitivity. In contrast, the geographic allocation of Puumala virus strains to defined outbreak regions in Germany was previously done based on S segment sequences. We show that the routinely generated partial L segment sequences resulting from the pan-hanta L-PCR assay provide sufficient phylogenetic signal to inform the molecular epidemiology of the Puumala virus. Consequently, an additional S segment analysis seems no longer necessary for the identification of the spatial origin of a virus strain.

摘要

为了筛选诊断标本中汉坦病毒基因组的存在或在自然界中鉴定新的汉坦病毒，pan-hanta L-PCR 检测法，一种针对 L 片段的广泛反应的嵌套逆转录聚合酶链反应（RT-PCR）检测法，因其通用性和高灵敏度而比其他检测法更受青睐。相比之下，以前德国根据 S 片段序列将普马拉病毒株分配到特定的暴发区域。我们表明，pan-hanta L-PCR 检测法产生的常规部分 L 片段序列提供了足够的系统发育信号，可用于告知普马拉病毒的分子流行病学。因此，对于鉴定病毒株的空间起源，额外的 S 片段分析似乎不再必要。