Dor Shlomit, Degani Ofir
Tel-Hai College, Upper Galilee, Tel-Hai 12210, Israel.
Migal-Galilee Research Institute, Tarshish 2, Kiryat Shmona 11016, Israel.
Plants (Basel). 2019 Jul 30;8(8):259. doi: 10.3390/plants8080259.
The fungus is a soil-borne, seed-borne vascular wilt pathogen that causes severe damage to sensitive L. (maize) hybrids throughout Egypt, Israel, India, Spain, and other countries. It can undergo virulence variations and survive as spores, sclerotia, or mycelia on plant residues. Maize, L. (lupine) and L. (cotton) are the only known hosts of . Identification of new plant hosts that can assist in the survival of the pathogen is an essential step in restricting disease outbreak and spread. Here, by field survey and growth chamber pathogenicity test, accompanied by real-time PCR analysis, the presence of the fungal DNA inside the roots of cotton (Pima cv.) plants was confirmed in infested soil. Moreover, we identified in (green foxtail) and (watermelon, Malali cv.). Infected watermelon sprouts had delayed emergence and development, were shorter, and had reduced root and shoot biomass. infection also affected root biomass and phenological development of cotton plants but caused only mild symptoms in green foxtail. No DNA was detected in (barley, Noga cv.) and the plants showed no disease symptoms except for reduced shoot weight. These findings are an important step towards uncovering the host range and endophytic behavior of , encouraging expanding this evaluation to other plant species.
这种真菌是一种土壤传播、种子传播的维管束萎蔫病原体，在埃及、以色列、印度、西班牙和其他国家对敏感的玉米杂交种造成严重损害。它会发生毒力变异，并以孢子、菌核或菌丝体的形式在植物残体上存活。玉米、羽扇豆和棉花是已知的该真菌仅有的寄主。鉴定有助于病原体存活的新植物寄主是限制疾病爆发和传播的关键一步。在此，通过田间调查和生长室致病性试验，并辅以实时PCR分析，在受侵染土壤中证实了棉花（皮马品种）植株根部存在真菌DNA。此外，我们在狗尾草和西瓜（马拉利品种）中鉴定出了该真菌。受感染的西瓜芽出苗和发育延迟，植株较矮，根和地上部生物量减少。该真菌感染也影响了棉花植株的根生物量和物候发育，但仅在狗尾草中引起轻微症状。在大麦（诺加品种）中未检测到该真菌DNA，除地上部重量减轻外，植株未表现出疾病症状。这些发现是揭示该真菌寄主范围和内生行为的重要一步，有助于推动将这种评估扩展到其他植物物种。
