Department of Science and Engineering of Oxide Materials and Nanomaterials, Politehnica University of Bucharest, 011061 Bucharest, Romania.
SC Microsin SRL, 032364 Bucharest, Romania.
Medicina (Kaunas). 2019 Jul 30;55(8):421. doi: 10.3390/medicina55080421.
Medical devices such as catheters are used on a large scale to treat heart and cardiovascular diseases. Unfortunately, they present some important drawbacks (structure failure, calcifications, infections, thrombosis, etc.), with the main side effects occurring due to adhesion and proliferation of bacteria and living cells on the surface of the implanted devices. The aim of this work is to modify the surface of polyvinyl chloride (PVC), an affordable biocompatible material, in order to reduce these aforementioned side effects. The surface of PVC was modified by depositing a thin layer also of PVC that incorporates an active substance, dicoumarol (a well-known anticoagulant), by spin coating process. The modified surfaces were analyzed by Fourier-transform infrared (FT-IR) microscopy, Fourier-transform infrared (FT-IR) spectroscopy, Ultraviolet-visible spectroscopy (UV-VIS), and Scanning electron microscopy (SEM) in order to determine the surface morphology and behavior. The samples were tested for Gram-positive ( ATCC 25923) and Gram-negative ( ATCC 27853) standard strains from American Type Culture Collection (ATCC). The material obtained had a smooth surface with a uniform distribution of dicoumarol, which is released depending on the deposition parameters. The concentration of dicoumarol at the surface of the material and also the release rate is important for the applications for which the surface modification was designed. PVC modified using the proposed method showed a good ability to prevent salt deposition and decreased the protein adhesion, and the resistance to bacterial adherence was improved compared with standard PVC.
医疗器械，如导管，被大规模用于治疗心脏和心血管疾病。不幸的是，它们存在一些重要的缺点（结构失效、钙化、感染、血栓形成等），主要的副作用是由于植入设备表面的细菌和活细胞的黏附和增殖引起的。本工作的目的是修饰聚氯乙烯（PVC）的表面，PVC 是一种经济实惠的生物相容性材料，以减少上述副作用。通过旋涂工艺在 PVC 表面沉积一层也包含活性物质双香豆素（一种众所周知的抗凝剂）的 PVC 薄层来修饰表面。通过傅里叶变换红外（FT-IR）显微镜、傅里叶变换红外（FT-IR）光谱、紫外可见光谱（UV-VIS）和扫描电子显微镜（SEM）对修饰后的表面进行分析，以确定表面形貌和行为。对来自美国典型培养物保藏中心（ATCC）的革兰氏阳性（ATCC 25923）和革兰氏阴性（ATCC 27853）标准菌株进行了测试。所获得的材料具有光滑的表面，双香豆素分布均匀，其释放取决于沉积参数。材料表面的双香豆素浓度和释放速率对于表面修饰设计的应用非常重要。与标准 PVC 相比，使用所提出的方法修饰的 PVC 显示出良好的防止盐沉积的能力，降低了蛋白质黏附性，并且细菌黏附的抵抗力得到了提高。
