State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, College of Agriculture and Biotechnology, Institute of Insect Sciences, Zhejiang University, 310029, Hangzhou, China.
Transgenic Res. 2012 Apr;21(2):279-91. doi: 10.1007/s11248-011-9530-x. Epub 2011 Jun 19.
In this study, the non-target effects of Bt rice "KMD2" expressing a Cry1Ab protein on the performance of the brown planthopper (BPH), Nilaparvata lugens, over multiple generations were evaluated under laboratory and field conditions. In the laboratory, BPH was reared to observe the impact of the Bt rice as compared to its parental non-Bt cultivar Xiushui 11, while the population dynamics and oviposition performance of BPH were investigated in the field. The survival of BPH nymphs fed Bt and non-Bt rice did not differ significantly. The nymph developmental duration of BPH was significantly delayed by the Bt rice by comparison with the non-Bt rice for the 1st and 2nd but not the 4th generation. Most importantly, the fecundity of BPH on the Bt rice was significantly decreased in every generation when compared with the non-Bt rice. In the field investigations, the population density of BPH nymphs was significantly lower in the Bt rice field. However, the temporal pattern of population dynamics of BPH adults was similar between the Bt and non-Bt rice, presumably due to migratory interference of the adults. In the Bt rice field, the percentage of tillers with eggs and the number of eggs per tiller were also significantly lower from tillering to mature stage. Additionally, Cry1Ab protein could not be detected in guts from single BPH adults. In general, our results suggest that the Bt rice "KMD2" could not stimulate an outbreak of BPH.
在本研究中，评估了转 Cry1Ab 蛋白 Bt 水稻“KMD2”对褐飞虱（Nilaparvata lugens）多世代非靶标效应。在实验室条件下，饲养褐飞虱以观察与亲本非 Bt 品种秀水 11 相比的 Bt 水稻的影响，同时在田间调查褐飞虱的种群动态和产卵性能。Bt 和非 Bt 水稻饲养的褐飞虱若虫存活率没有显著差异。与非 Bt 水稻相比，Bt 水稻第 1 和第 2 代但不是第 4 代褐飞虱若虫的发育时间显著延迟。最重要的是，与非 Bt 水稻相比，褐飞虱在每一代的 Bt 水稻上的繁殖力都显著降低。在田间调查中，Bt 水稻田的褐飞虱若虫种群密度显著降低。然而，Bt 和非 Bt 水稻上的褐飞虱成虫种群动态的时间模式相似，可能是由于成虫的迁移干扰。在 Bt 水稻田，从分蘖到成熟阶段，带卵分蘖的比例和每分蘖的卵数也显著降低。此外，单个褐飞虱成虫的肠道中未检测到 Cry1Ab 蛋白。总的来说，我们的结果表明，Bt 水稻“KMD2”不能刺激褐飞虱的爆发。
