Laboratory of Biochemistry, Faculty of Pharmacy, Osaka Ohtani University.
Laboratory of Molecular Biology, Faculty of Pharmacy, Osaka Ohtani University.
Biol Pharm Bull. 2019;42(8):1295-1302. doi: 10.1248/bpb.b18-00984.
Obesity is characterized by abnormal or excessive fat accumulation, which leads to the development of metabolic syndrome. Because oxidative stress is increased in obesity, antioxidants are regarded as suitable agents for preventing metabolic syndrome. Here, we examined the impact of cranberry, which contains various antioxidants, on metabolic profiles, including that during the progression of non-alcoholic fatty liver disease (NAFLD), in high-fat diet (HFD)-fed C57BL/6 mice. We observed that oxidative stress was diminished in mice that were fed HFD diets supplemented with 1 and 5% cranberry powder as compared with that in HFD-fed control mice. Notably, from 1 week after beginning the diets to the end of the study, the body weight of mice in the cranberry-treatment groups was significantly lower than that of mice in the HFD-fed control group; during the early treatment phase, cranberry suppressed the elevation of serum triglycerides; and adipocytes in the adipose tissues of cranberry-supplemented-HFD-fed mice were smaller than these cells in HFD-fed control mice. Lastly, we examined the effect of cranberry on NAFLD, which is one of the manifestations of metabolic syndrome in the liver. Histological analysis of the liver revealed that lipid-droplet formation and hepatocyte ballooning, which are key NAFLD characteristics, were both drastically decreased in cranberry-supplemented-HFD-fed mice relative to the levels in HFD-fed control mice. Our results suggest that cranberry ameliorates HFD-induced metabolic disturbances, particularly during the early treatment stage, and exhibits considerable potential for preventing the progression of NAFLD.
肥胖的特征是异常或过度的脂肪积累，这导致了代谢综合征的发展。由于肥胖症中氧化应激增加，因此抗氧化剂被认为是预防代谢综合征的合适药物。在这里，我们研究了蔓越莓的作用，蔓越莓含有各种抗氧化剂，对代谢谱的影响，包括在非酒精性脂肪性肝病（NAFLD）进展过程中，高脂肪饮食（HFD）喂养的 C57BL / 6 小鼠。我们观察到，与 HFD 喂养的对照组相比，用 1％和 5％蔓越莓粉补充 HFD 的饮食喂养的小鼠的氧化应激减轻了。值得注意的是，从开始饮食的第 1 周到研究结束，补充蔓越莓的饮食组的小鼠体重明显低于 HFD 喂养的对照组；在早期治疗阶段，蔓越莓抑制了血清甘油三酯的升高；并且补充 HFD 的蔓越莓喂养的小鼠的脂肪组织中的脂肪细胞比 HFD 喂养的对照组的细胞小。最后，我们检查了蔓越莓对 NAFLD 的影响，NAFLD 是肝脏代谢综合征的表现之一。肝脏的组织学分析表明，与 HFD 喂养的对照组相比，补充 HFD 的蔓越莓喂养的小鼠中的脂质滴形成和肝细胞气球样变性这两个关键的 NAFLD 特征都明显减少。我们的结果表明，蔓越莓可改善 HFD 引起的代谢紊乱，尤其是在早期治疗阶段，并且对预防 NAFLD 的进展具有很大的潜力。
