Effects of amine fluoride and probiotic mouthwash on levels of in orthodontic patients: A randomized controlled trial.

INTRODUCTION

is one among the major etiological agent in periodontal diseases and has been proved to cause gingival inflammation under orthodontic appliances.

AIM

To assess the effect of amine fluoride and probiotic mouthwashes on levels of during orthodontic treatment, using real time-polymerase chain reaction (RT-PCR).

MATERIALS AND METHODS

A randomised controlled trial was performed including 45 patients. There were three groups: Group A (control group), Group B (patients using Amflor mouthwash) and Group C (patients using probiotic mouthwash). During the treatment, all the 3 groups were advised to brush twice a day using Colgate tooth paste and Group B patients were advised to swish 15 ml fluoridated mouthwash (Amflor) and Group C advised to swish probiotic for 60 s before swallowing, once in the morning and once before sleep. After gently removing supragingival plaque, subgingival plaque samples were collected by inserting a sterile dental curette into the bottom of the gingival crevice around 31, 32, 41, and 42 at two different intervals: T1 at start and T2 after 6 months of fixed orthodontic treatment. Sub-gingival plaque samples collected were transferred to laboratory within 48 h for RT-PCR analysis in Tris-buffer solution, to maintain the integrity of bacterial DNA.

RESULTS

The levels of were significantly decreased with probiotic mouth wash.

CONCLUSIONS

Probiotic mouth wash can be used as an adjunctive measure along with regular brushing to improve periodontal status during fixed orthodontic treatment.