Mohamed Hanan R H, Welson Mary, Yaseen Ahmed Essa, El-Ghor Akmal
Zoology Department, Faculty of Science, Cairo University, Giza, Egypt.
Zoology Department, Faculty of Science, Suez University, Suez, Egypt.
Drug Chem Toxicol. 2021 Nov;44(6):631-641. doi: 10.1080/01480545.2019.1643876. Epub 2019 Aug 1.
The unique physicochemical properties of graphene oxide (GO) nanoparticles increase their uses in a wide range of applications that increase their release into the environment, and thus human exposure. However, the clastogenicity and genotoxicity of GO nanoparticles have not been well investigated. The current study was, therefore, designed to investigate the possible induction of chromosomal and DNA damage by GO nanoparticles and their impact on the tissue architecture in mice. Oral administration of GO nanoparticles for one or five consecutive days at the three dose levels 10, 20 or 40 mg/kg significantly increased the micronuclei and DNA damage levels in a dose-dependent manner in mice bone marrow cells, as well as caused, histological lesions including apoptosis, necrosis, inflammations and cells degeneration in the mice liver and brain tissue sections compared to the normal control mice. Thus, we concluded that oral administration of GO nanoparticles induced chromosomal and DNA damage in a dose-dependent manner as well as histological injuries in both acute and subacute treatments.
氧化石墨烯（GO）纳米颗粒独特的物理化学性质增加了它们在广泛应用中的使用，这也增加了它们向环境中的释放，进而增加了人类接触的机会。然而，GO纳米颗粒的断裂形成性和基因毒性尚未得到充分研究。因此，本研究旨在调查GO纳米颗粒是否可能诱导染色体和DNA损伤以及它们对小鼠组织结构的影响。以10、20或40mg/kg这三个剂量水平连续1天或5天口服给予GO纳米颗粒，与正常对照小鼠相比，显著增加了小鼠骨髓细胞中的微核和DNA损伤水平，并且在小鼠肝脏和脑组织切片中引起了包括凋亡、坏死、炎症和细胞变性在内的组织学损伤。因此，我们得出结论，口服给予GO纳米颗粒在急性和亚急性处理中均以剂量依赖性方式诱导染色体和DNA损伤以及组织学损伤。
