Division of Biotechnology, Food Technology, Graduate School, Korea University, Seoul, Republic of Korea.
Research Affairs, Korea University Anam Hospital, Korea University College of Medicine, Seoul, Republic of Korea.
J Sci Food Agric. 2019 Dec;99(15):6806-6813. doi: 10.1002/jsfa.9965. Epub 2019 Sep 5.
Enzymatic hydrolysis and high hydrostatic pressure (HHP) are common processing techniques in the extraction of active compounds from food materials. The aim of this study was to investigate the effects of enzymatic hydrolysis combined with HHP treatments on ginsenoside metabolites in red ginseng.
The yield and changes in the levels of polyphenol and ginsenoside were measured in red ginseng treated with commercial enzymes such as Ultraflo L, Viscozyme, Cytolase PCL5, Rapidase and Econase E at atmospheric pressure (0.1 MPa), 50 MPa, and 100 MPa. β-Glucosidase activity of Cytolase was the highest at 4258.2 mg , whereas Viscozyme showed the lowest activity at 10.6 mg . Pressure of 100 MPa did not affect the stability or the activity of the β-glucosidase. Treatment of red ginseng with Cytolase and Econase at 100 MPa significantly increased the dry weight and polyphenol content of red ginseng, compared with treatments at 0.1 MPa and 50 MPa (P < 0.05). The amounts of ginsenoside and ginsenoside metabolites derived from red ginseng processed using Cytolase were higher than those derived from red ginseng treated with the other enzymes. Treatment with Cytolase also significantly increased the skin and intestinal permeability of red ginseng-derived polyphenols.
Cytolase could be useful as an enzymatic treatment to enhance the yield of bioactive compounds from ginseng under HHP. In addition, ginsenoside metabolites obtained by Cytolase hydrolysis combined with HHP are functional substances with increased intestinal and skin permeability. © 2019 Society of Chemical Industry.
酶解和高静压（HHP）是从食品原料中提取活性化合物的常见加工技术。本研究旨在探讨酶解与 HHP 联合处理对红参中人参皂苷代谢物的影响。
在大气压（0.1 MPa）、50 MPa 和 100 MPa 下，用商业酶如 Ultraflo L、Viscozyme、Cytolase PCL5、Rapidase 和 Econase E 处理红参，测量了红参的产率和多酚和人参皂苷水平的变化。Cytolase 的β-葡萄糖苷酶活性最高，为 4258.2 mg，而 Viscozyme 的活性最低，为 10.6 mg。100 MPa 的压力不会影响β-葡萄糖苷酶的稳定性或活性。与在 0.1 MPa 和 50 MPa 下处理相比，用 Cytolase 和 Econase 在 100 MPa 下处理红参可显著增加红参的干重和多酚含量（P<0.05）。用 Cytolase 处理的红参产生的人参皂苷和人参皂苷代谢物的量高于用其他酶处理的红参。Cytolase 处理还显著提高了红参来源多酚的皮肤和肠道通透性。
Cytolase 可作为一种酶处理方法，在 HHP 下提高人参中生物活性化合物的产量。此外，通过 Cytolase 水解与 HHP 结合获得的人参皂苷代谢物是具有增加肠道和皮肤通透性的功能物质。© 2019 化学工业协会。
