Kim Eun Hye, Lim Seokwon, Kim Sang Oh, Ahn Sung Ho, Choi Young Jin
Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea.
Biosci Biotechnol Biochem. 2013;77(5):1138-40. doi: 10.1271/bbb.120823. Epub 2013 May 7.
Ginsenoside 20-O-β-D glucopyranosyl-20(S)-protopanaxadiol (compound K), a minor ginsenoside, is not found in white raw ginseng, but has better bioavailability than the major ginsenosides in ginseng. Employing commercial enzyme packages for industrial applications, the optimum conditions for enzymatic transformation for the highest content of compound K was explored to enhance the health benefits of ginseng extract. Cytolase PCL 5 was selected from commercial enzyme packages nominated for high β-glucosidase activity. By response surface methodology, the optimal conditions were identified as 78 h of treatment at pH 4.3 at 55.4 °C for 2.068 mg/mL of compound K, showing good agreement with the experimental value.
人参皂苷20 - O-β - D - 吡喃葡萄糖基 - 20(S) - 原人参二醇（化合物K）是一种微量人参皂苷，在白参中不存在，但比人参中的主要人参皂苷具有更好的生物利用度。为了提高人参提取物的健康益处，利用商业酶制剂进行工业应用，探索了使化合物K含量最高的酶促转化的最佳条件。从具有高β - 葡萄糖苷酶活性的商业酶制剂中选择了Cytolase PCL 5。通过响应面法，确定最佳条件为在55.4℃、pH 4.3下处理78小时，化合物K含量为2.068mg/mL，与实验值吻合良好。
