Graduate School of International Agricultural Technology, Seoul National University, Pyeongchang-gun, Gangwon-do, 25354, Republic of Korea.
Institute of Food Industrialization, Institutes of Green Bioscience & Technology, Center for Food and Bioconvergece, Seoul National University, Pyeongchang-gun, Gangwon-do, 25354, Republic of Korea.
Appl Microbiol Biotechnol. 2024 Feb 14;108(1):207. doi: 10.1007/s00253-024-13028-2.
Compound K (CK), a ginsenoside with high bioavailability, is present at low levels in wild-simulated ginseng leaves (WSGL). WSGL contains the CK precursors, Rd and F2, in amounts up to 26.4 ± 0.4 and 24.1 ± 1.9 mg/g extract, respectively. In this study, CK production in WGSL reached 25.9 ± 1.0 mg/g extract following treatment with Viscozyme, Celluclast 1.5 L, Pectinex Ultra SP-L, and their combination. The antioxidant activities indicated by oxygen radical absorbance capacity, ferric reducing antioxidant power, and ABTS- and DPPH radical scavenging activity of enzyme-treated WSGL were enhanced 1.69-, 2.51-, 2.88-, and 1.80-fold, respectively, compared to non-treated WSGL. Furthermore, the CK-enriched WSGL demonstrated a 1.94-fold decrease in SA-β-galactosidase expression in human dermal fibroblasts and a 3.8-fold enhancement of inhibition of nitric oxide release in lipopolysaccharide-induced RAW 264.7 cells relative to non-treated WSGL. Consequently, WSGL subjected to enzymatic upcycling has potential as a functional material in the food and pharmaceutical industries.
化合物 K（CK）是一种生物利用度高的人参皂苷，在野生模拟人参叶（WSGL）中的含量较低。WSGL 中含有 CK 的前体 Rd 和 F2，其含量分别高达 26.4±0.4 和 24.1±1.9 mg/g 提取物。在这项研究中，用 Viscozyme、Celluclast 1.5L、Pectinex Ultra SP-L 及其组合处理 WSGL 后，CK 的产量达到 25.9±1.0 mg/g 提取物。经酶处理的 WSGL 的氧自由基吸收能力、铁还原抗氧化能力、ABTS 和 DPPH 自由基清除活性分别比未经处理的 WSGL 提高了 1.69 倍、2.51 倍、2.88 倍和 1.80 倍。此外，与未经处理的 WSGL 相比，富含 CK 的 WSGL 使人类真皮成纤维细胞中 SA-β-半乳糖苷酶的表达降低了 1.94 倍，脂多糖诱导的 RAW 264.7 细胞中一氧化氮释放的抑制作用增强了 3.8 倍。因此，经过酶法升级处理的 WSGL 有望成为食品和制药行业的功能性材料。
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