Am J Respir Cell Mol Biol. 2019 Aug;61(2):150-161. doi: 10.1165/rcmb.2019-0191ST.
Defining responses of the structural and immune cells in biologic systems is critically important to understanding disease states and responses to injury. This requires accurate and sensitive methods to define cell types in organ systems. The principal method to delineate the cell populations involved in these processes is flow cytometry. Although researchers increasingly use flow cytometry, technical challenges can affect its accuracy and reproducibility, thus significantly limiting scientific advancements. This challenge is particularly critical to lung immunology, as the lung is readily accessible and therefore used in preclinical and clinical studies to define potential therapeutics. Given the importance of flow cytometry in pulmonary research, the American Thoracic Society convened a working group to highlight issues and technical challenges to the performance of high-quality pulmonary flow cytometry, with a goal of improving its quality and reproducibility.
定义生物系统中结构和免疫细胞的反应对于理解疾病状态和对损伤的反应至关重要。这需要准确和敏感的方法来定义器官系统中的细胞类型。描绘这些过程中涉及的细胞群体的主要方法是流式细胞术。尽管研究人员越来越多地使用流式细胞术，但技术挑战会影响其准确性和可重复性，从而极大地限制了科学进展。由于流式细胞术在肺部免疫学中的重要性，美国胸科学会召集了一个工作组，重点介绍了高性能肺部流式细胞术的性能问题和技术挑战，目标是提高其质量和可重复性。
