Zhai Jia Y, Qi Peng Z, Yang Xiao A, Ren Zhong J, Zhang Zi H, Gao Jia X, Zhu Deng H, Fu Pei P
National Engineering Research Center of Marine Facilities Aquaculture, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, Zhejiang, P. R. China.
Taizhou Key Laboratory of Biomedicine and Advanced Dosage Forms, School of Life Sciences, Taizhou University, Taizhou, Zhejiang, P. R. China.
Parasitology. 2024 Sep;151(10):1104-1107. doi: 10.1017/S0031182024000702. Epub 2024 Nov 21.
During the investigation of parasitic pathogens of , infection of a -like protozoan parasite was detected by alternative Ray's Fluid Thioglycolate Medium (ARFTM). The diameter of hypnospores or prezoosporangia was 8–27 (15.6 ± 4.0, = 111) μm. The prevalence of the -like species in was 25 and 12.5% using ARFTM and PCR, respectively. The ITS1-5.8S-ITS2 fragments amplified by PCR assay had 100% homology to that of , suggesting that the protozoan parasite was and was its new host in East China Sea (ECS). Histological analysis showed the presence of trophozoites of in gill, mantle and visceral mass, and the schizonts only found in visceral mass. infection led to inflammatory reaction of hemocyte and the destruction of digestive tubules in visceral mass, which had negative effect on health of the farmed and it deserves more attention.
在对[具体生物名称]寄生虫病原体的调查过程中，通过改良雷氏硫乙醇酸盐培养基（ARFTM）检测到一种类[具体生物名称]原生动物寄生虫的感染。休眠孢子或前游动孢子囊的直径为8 - 27（15.6 ± 4.0，n = 111）μm。使用ARFTM和PCR方法，该类[具体生物名称]物种在[具体生物名称]中的感染率分别为25%和12.5%。通过PCR检测扩增的ITS1 - 5.8S - ITS2片段与[具体生物名称]的片段具有100%的同源性，这表明该原生动物寄生虫为[具体生物名称]，且[具体生物名称]是其在东海的新宿主。组织学分析显示，在鳃、外套膜和内脏团中存在[具体生物名称]的滋养体，而裂殖体仅在内脏团中发现。[具体生物名称]感染导致血细胞发生炎症反应，并破坏内脏团中的消化小管，这对养殖的[具体生物名称]健康产生负面影响，值得更多关注。
