National Citrus Engineering Research Center, Citrus Research Institute, Southwest University, Chongqing 400712, China.
Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China.
Viruses. 2019 Jul 31;11(8):696. doi: 10.3390/v11080696.
An emerging virus-like flower yellowing disease (FYD) of green Sichuan pepper ( v. ) has been recently reported. Four new RNA viruses were discovered in the FYD-affected plant by the virome analysis using high-throughput sequencing of transcriptome and small RNAs. The complete genomes were determined, and based on the sequence and phylogenetic analysis, they are considered to be new members of the genera (), (unassigned), (), and (), respectively. Therefore, the tentative names corresponding to these viruses are green Sichuan pepper-nepovirus (GSPNeV), -idaeovirus (GSPIV), -enamovirus (GSPEV), and -nucleorhabdovirus (GSPNuV). The viral population analysis showed that GSPNeV and GSPIV were dominant in the virome. The small RNA profiles of these viruses are in accordance with the typical virus-plant interaction model for . Rapid and sensitive RT-PCR assays were developed for viral detection, and used to access the geographical distributions. The results revealed a correlation between GSPNeV and the FYD. The viruses pose potential threats to the normal production of green Sichuan pepper in the affected areas due to their natural transmission and wide spread in fields. Collectively, our results provide useful information regarding taxonomy, transmission and pathogenicity of the viruses as well as management of the FYD.
一种新出现的类似病毒的花椒黄花病（FYD）最近有报道。通过对转录组和小 RNA 的高通量测序进行病毒组分析，在受 FYD 影响的植物中发现了四种新的 RNA 病毒。确定了完整的基因组，并根据序列和系统发育分析，它们分别被认为是属（）、（未分配）、（）和（）的新成员。因此，这些病毒的暂定名称分别为花椒芜菁花叶病毒（GSPNeV）、-杆状病毒（GSPIV）、-腰果病毒（GSPEV）和-核衣壳病毒（GSPNuV）。病毒群体分析表明，GSPNeV 和 GSPIV 在病毒组中占优势。这些病毒的小 RNA 图谱与典型的病毒-植物相互作用模式一致。开发了用于病毒检测的快速灵敏 RT-PCR 检测方法，并用于评估地理分布。结果表明 GSPNeV 与 FYD 之间存在相关性。由于这些病毒的自然传播和在田间的广泛传播，它们对受影响地区花椒的正常生产构成了潜在威胁。总的来说，我们的研究结果为这些病毒的分类学、传播和致病性以及 FYD 的管理提供了有用的信息。
