Micalis Institute, Université Paris-Saclay, INRAE, AgroParisTech, Jouy-en-Josas, France.
Biotechnological Processes Unit, IMDEA Energy, Avenida Ramón De La Sagra, 3. 28935, Móstoles, Madrid, Spain.
FEMS Yeast Res. 2021 Sep 11;21(6). doi: 10.1093/femsyr/foab047.
In recent years, there has been a growing interest in the use of renewable sources for bio-based production aiming at developing sustainable and feasible approaches towards a circular economy. Among these renewable sources, organic wastes (OWs) can be anaerobically digested to generate carboxylates like volatile fatty acids (VFAs), lactic acid, and longer-chain fatty acids that are regarded as novel building blocks for the synthesis of value-added compounds by yeasts. This review discusses on the processes that can be used to create valuable molecules from OW-derived VFAs; the pathways employed by the oleaginous yeast Yarrowia lipolytica to directly metabolize such molecules; and the relationship between OW composition, anaerobic digestion, and VFA profiles. The review also summarizes the current knowledge about VFA toxicity, the pathways by which VFAs are metabolized and the metabolic engineering strategies that can be employed in Y. lipolytica to produce value-added biobased compounds from VFAs.
近年来，人们对利用可再生资源进行基于生物的生产越来越感兴趣，旨在开发可持续和可行的方法来实现循环经济。在这些可再生资源中，有机废物 (OWs) 可以进行厌氧消化，生成羧酸，如挥发性脂肪酸 (VFAs)、乳酸和长链脂肪酸，这些酸被认为是酵母合成增值化合物的新型构建块。本综述讨论了可用于从 OW 衍生的 VFAs 中制造有价值分子的过程；产油酵母解脂耶氏酵母 (Yarrowia lipolytica) 直接代谢此类分子所采用的途径；以及 OW 组成、厌氧消化和 VFA 谱之间的关系。该综述还总结了目前关于 VFA 毒性、VFA 代谢途径以及可以在解脂耶氏酵母中采用的代谢工程策略的知识，这些策略可用于从 VFAs 生产增值的生物基化合物。
